Treating Slides with ConcanavalinA
1) ConcanavalinA is stored in the -20°C freezer in the box labeled “ConcanavalinA.” Allow it to thaw.
2) Clean glass microscope slides with 95% ethanol and a kimwipe. Clean coverslips as well. Only handle the edges.
3) Add 5 μL of ConA solution to slide, spreading around with pipette tip. Place slides in a container to protect from dust. Allow to dry for approximately 20 minutes
4) Once dry, add 3 μL cell suspension.
5) Place coverslips over slides, pressing down slightly
6) Seal cover slip with clear nail polish
Protocol adapted from Lucy F. Pemberton
Pemberton L.F. (2014) Preparation of Yeast Cells for Live-Cell Imaging and Indirect Immunofluorescence. In: Smith J., Burke D. (eds) Yeast Genetics. Methods in Molecular Biology (Methods and Protocols), vol 1205. Humana Press, New York, NY