Table of Contents
0.5M EDTA
— Christine Robbins 2018/05/01 12:40
Description
0.5M EDTA is used to make 50x TAE and 10x TE Buffer.
Location:Shelf L
Media Recipe and Directions
Directions
- Grab a beaker of the appropriate size and add a stir bar
- Add about 1/4 of the final volume of miliQ Water
- Add the appropriate amount of EDTA and the NaOH.
- Adjust volume to about 100mL below your final volume
- Adjust the pH of your solution to 8 using 50% NaOH. Your solution will not fully dissolve until you reach pH 8.
- Add milliQ Water to meet your final volume
- Transfer the solution into labeled bottles
- Autoclave
Media Recipe