McCormick Lab Wiki

To perform DNA extraction on MEF cells, use QuickExtract. QuickExtract will detach and destroy the adherent MEFs cultured on a tissue culture plate, and after this, ~10-minute protocol, the DNA in the mixture will be ready for PCR.

1. Culture MEFs in a 96-well or a 24-well plate. Using larger plates will result in the use of more reagents but is doable as well.
2. Retrieve QuickExtract aliquot from the -20 freezer, adjust 42*C heat block to 65*C.
3. In the BSL-2 hood, aspirate off media
4. Add Enough QuickExtract to the well to cover the plate's surface area
5. With a micropipette, scrape the plate to ensure the removal and destruction of the MEF cells. Mix the QuickExtract liquid around the plate with the pipette.
6. Move the liquid into a microcentrifuge tube.
7. Vortex for 15 seconds.
8. Place on 65*C heat block for 6 min.
9. Vortex for 15 seconds.
10. Place on 95*C heat block for 2 min.
11. Once cooled to room temperature, your sample is now ready to PCR.