Table of Contents
50% PEG
— Christine Robbins 2018/10/01 15:22
Description
50% PEG is used in a number of transformation protocols including episomal transformation and high efficiency transformations.
Location:Shelf L
Warnings This is very viscous! Pipette slowly.
Media Recipe and Directions
Directions
- Add the appropriate amount of PEG to the beaker and mix until dissolved
- Add water to the final volume
- Filter sterilize into labeled bottles
Media Recipe
| Reagents | Final Volume | ||
|---|---|---|---|
| 100mL | 250mL | 500L | |
| PEG | 50g | 125g | 250g |