Table of Contents
Pulling Bacterial Strains
— Ryla Cantergiani 2020/04/13 14:38
Overview
We have our bacterial strains frozen in the -80C freezer. When you need to use a strain, you'll have to pull it and grow it up first. The bacteria will take time to grow up, so plan ahead when organizing your experiments.
Materials
| Materials |
|---|
| frozen bacterial strain |
| LB (liquid or plates) |
| pipette tips |
Procedure
- Check the MM-BACTERIA-STRAINS spreadsheet to find the MM# of your bacteria
- Depending on what you need the bacteria for, you can grow it up on LB plates or in LB liquid media. Labels your plates/tubes.
- Ethanol and wipe down your bench. Turn on a flame.
- Using the polar bear gloves, get your bacterial strain out of the -80C freezer.
- Using a pipette tip (P10 or P20 work best), get a small bit of bacteria out of the tube, and gently place it onto your plate/into your liquid LB. If using a plate, let the bacteria dry before streaking out. If using liquid LB, vortex to mix.
- Place in the 37C incubator to grow. Plates can go in the 37 in lab. Tubes need to go in the shaking 37C in CRF.