Streaking Yeast Plates:
1) Clean benchtop with 70% ethanol and start Bunsen Burner.
2) Label YPD plates appropriately.
3) Pull appropriate yeast strains from freezer (see protocol on Wiki).
4) Open strain tube and scrape a pen-tip sized cluster of yeast with a sterile pipet tip.
5) Gently dab cell cluster onto region of labeled plate shown in green. Replace plate lid and allow to dry.
6) After yeast has dried, streak with flat part of toothpick or pipet tip as shown in blue.
7) With a new toothpick or pipet tip, streak as shown in red, crossing the first and second streaks only once as shown above.
8) Repeat 7 for region shown in purple.
9) Parafilm plates
10) Place plates in 30 Degree Celsius incubator, turn off flame, and clean benchtop.