M9 solution is used when working with worms. It is used in Freezing Worm Strains.

[Insert image of media here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of “nameofmedia-image.png” . Select “Add images and other files” from above and upload your image. When the Link Settings tab pops up, select “Direct link to original”, “Align the image on the right” and “Medium Version”, then click insert. You may have to mess with the size. ]

Location:Shelf ???

[Insert Image of lab location here. To do this, take the template of the lab, that is in the wiki folder, and add a red dot in the location of the reagent using InkScape. Export the image to the dropbox folder 'mccormicklab1>Wiki-Documents and Figures> Pictures for Wiki….> Reagents' With the file name of “nameofmedia-map.png”. Then, add the image by selecting “Add images and other files” from above and upload your image. When the Link Settings tab pops up, select “Direct link to original”, “Align the image on the right” and “Medium Version”, then click insert. ]

Notes and Warning: M9 should be clear in appearance; if cloudy or otherwise unusual, make a new stock.

Directions - 1 L

1. Label a 1L beaker with media, initials, and date. Add a stir bar.
2. Fill with 1/4 of the MilliQ H2O
3. Add Na2HPO4*7H2O, KH2PO4, NaCl, and MgSO4*7H2O. Stir until all solid has dissolved.
4. Pour into labeled bottles. Autoclave.
5. Please include all appropriate directions for the type of media you are making. This will include what type of glassware is needed (ex: a flask at least 2x the volume of the final volume of media you are making), stir bars, stir plate, if the media needs to be pH-ed, autoclaving or filter sterilization, and anything else you can think of that will be useful for the next person making this type of media.

Media Recipe

Storage instructions

Store in bottles at room temperature.

Na2HPO4*7H2O P/N: Sigma-Aldrich S9390-1KG

KH2PO4 P/N: VWR BDH0268-5006

NaCl P/N: EMD SX0420-3

MgSO4*7H2O P/N: Bioworld 413000-40-1

Millipore filter P/N: Millipore SCGP505RE (<0.22um) and bottle.