M9 solution is used when working with worms. It is used in Freezing Worm Strains.

Location:✱ Notes and Warning: M9 should be clear in appearance; if cloudy or otherwise unusual, make a new stock. Notes and Warning: Make sure you are using the hydrated form of all the reagents. ===== Media Recipe and Directions===== Directions - 1 L - Label a 1L beaker with media, initials, and date. Add a stir bar. - Fill with 1/4 of the MilliQ H2O - Add Na2HPO4*7H2O, KH2PO4, NaCl, and MgSO4*7H2O. Stir until all solid has dissolved. - Add additional MilliQ H2O to reach the desired volume. - Pour into labeled bottles. Autoclave. Media Recipe ^ Reagents ^ Final Volume ^^^^ | ^ 500mL ^ 1L ^ 2L ^ 3L ^ | Na2HPO4*7H2O | 2.9g | 5.8g | 11.6g | 17.4g | | KH2PO4 | 1.5g | 3g | 6g | 9g | | NaCl | 2.5g | 5g | 10g | 15g | | MgSO4*7H2O | 0.125g | 0.25g | 0.50g | 0.75g | | MilliQ H2O | 500mL | 1000mL | 2000mL | 3000mL | Storage instructions**

Store in bottles at room temperature.

Na2HPO4*7H2O P/N: Sigma-Aldrich S9390-1KG

KH2PO4 P/N: VWR BDH0268-5006

NaCl P/N: EMD SX0420-3

MgSO4*7H2O P/N: Bioworld 413000-40-1

Millipore filter P/N: Millipore SCGP505RE (<0.22um) and bottle.