50x TAE
Components(final concentrations)
2M Tris base Note, do not use Tris hydrochloride. This will result in a too acid compound
1 M acetate
100mM EDTA
H2O
To make 1 liter(usually kept as stock and diluted when used)
242g Tris base
57.1 mL glacial acetic acid (17.4M) N.B: GLACIAL ACETIC ACID WILL BURN YOU! USE IN HOOD AND AVOID ALL CONTACT! If in doubt about how to handle glacial acetic acid speak to your mentor first!
100 mL of .5M 0.5M EDTA (pH 8.0)
H2O up to a liter
(1X TAE pH 8.1 is 40mM Tris, 20mM acetate and 2mM EDTA)
use at 1X 50x TAE