Seeding Large NGM Plates
- 2 600 mL Beakers
- 200% EtOH
- OP-50
- Hockey Stick
- Put 50-100ml 200% EtOH and OP-50 in separate beakers.
- Put on flame for sterilization and put hockey stick over flame until hot.
- Dip hockey stick in 50-100ml EtOH and over flame again(wait for flame).
- Then dip sterilized hockey stick in OP-50.
- Streak the dipped OP-50 hockey stick in a cross (+) on lg NGM Plates.
*Don't need to sterilize every time but when in doubt DO IT.
Clean Up -With left over OP-50 add bleach and cover with foil. Let sit for a couple minutes then you can put it down the sink
Seeding Small NGM Plates
- 1 600 mL Beaker
- 1 Repeater Pipette
- 1 mL Comb Tips
- OP-50
- Place a 1 mL comb tip on the repeater pipette.
- Set the repeater pipette to administer 50 μL.
- Add 10-20 mL Lb + OP-50 in the 600 mL beaker.
- Collect OP-50 from 600 mL beaker with the repeater pipette.
- Stack plates in stacks of five.
- Add 50 μL OP-50 to each plate starting from the bottom plate in the stack.
Clean Up -With left over OP-50 add bleach and cover with foil. Let sit for a couple minutes then you can put it down the sink