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LB LIQUID MEDIA MAKES 1 LITER
1) Retrieve a 2-liter beaker and add about 250 ml of milliQ water
2) Add a magnetic stir bar and place on the magnetic stirrer/hot plate. Turn on the stirrer with no heat.
3) Add the following reagents to the flask:
10 g Bacto Tryptone 5 g Bacto Yeast Extract 5 g NaCl 160 µl 50% NaOH solution *WEAR GLOVES*
When NaOH stock runs out, this could be made by adding 50 grams of water to 50 grams of sodium hydroxide pellets to make 50% w/v NaOH solution.
4) Bring the volume up to 1 liter with H2O
OPTIONAL: You may be planning to aliquot this now to smaller bottles in order to autoclave in place. 500ml bottles are a great size; be sure the undissolved media is very well resuspended when pouring.
5) Autoclave to sterilize