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LB BACTERIAL MEDIA
Makes 2 Liters
Recipe:
20 grams Bacto Tryptone 10 grams Yeast Extract 10 grams NaCl 320 microliters 50% NaOH 2 Liters milliQ Water
Materials:
GLOVES [3] Medium Weigh Boats [3] Sterile Spatulas [1] Sterile 2-Liter Beaker [1] Metric Scale [1] Magnetic Stirrer [1] Sterile Stir Bar [1] P-1000 Pipet [1] Sterile P-1000 Pipet Tip [5] Sterile 500-milliliter Bottles Autoclave Tape
1) Retrieve 2-Liter Beaker, place on magnetic stirrer, and add stir bar.
2) Obtain 500 milliliters milliQ Water and add to beaker.
3) With clean Weigh Boat, mass out 20 grams Bacto Tryptone and add slowly to Beaker. Allow complete mixing.
4) With clean Weigh Boat, mass out 10 grams Yeast Extract and add slowly to Beaker. Allow complete mixing.
5) With clean Weigh Boat, mass out 10 grams NaCl and add to Beaker.
6) [*GLOVES*] With P-1000 Pipet set to 320 microliters, pipet 320 microliters 50% NaOH into beaker.
7) While media mixes, label 5 500-milliliter Bottles with media type, date, and initials.
8) When media had completely mixed, fill Bottles with 400 milliliters of media each.
9) Loosely cap Bottles and mark with Autoclave Tape.
10) Autoclave bottles on liquid setting.
11) When autoclave cycle is complete, secure Bottle caps and store media.