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lb_meida_and_variations [2018/08/20 16:16] marissa_westenskow FORMAT |
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| - | LB LIQUID MEDIA | ||
| - | MAKES 1 LITER | ||
| - | 1) Retrieve a 2-liter beaker and add about 250 ml of milliQ water | ||
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| - | 2) Add a magnetic stir bar and place on the magnetic stirrer/hot plate. Turn on the stirrer with no heat. | ||
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| - | 3) Add the following reagents to the flask: | ||
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| - | 10 g Bacto Tryptone | ||
| - | 5 g Bacto Yeast Extract | ||
| - | 5 g NaCl | ||
| - | 160 µl 50% NaOH solution *WEAR GLOVES* | ||
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| - | When NaOH stock runs out, this could be made by adding 50 grams of water to 50 grams of sodium hydroxide pellets (VWR 1310-73-2) to make 50% w/v NaOH solution. | ||
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| - | 4) Bring the volume up to 1 liter with H2O | ||
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| - | OPTIONAL: You may be planning to aliquot this now to smaller bottles in order to autoclave in place. 500ml bottles are a great size; be sure the undissolved media is very well resuspended when pouring. | ||
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| - | 5) Autoclave to sterilize | ||