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lb_media_and_variations [2018/11/14 18:23] dan_felker |
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| - | __**LB BACTERIAL MEDIA**__ | ||
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| - | //Makes 2 Liters// | ||
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| - | Recipe: | ||
| - | 20 grams Bacto Tryptone | ||
| - | 10 grams Yeast Extract | ||
| - | 10 grams NaCl | ||
| - | 320 microliters 50% NaOH | ||
| - | 2 Liters milliQ Water | ||
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| - | Materials: | ||
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| - | [3] Medium Weigh Boats | ||
| - | [3] Sterile Spatulas | ||
| - | [1] Sterile 2-Liter Beaker | ||
| - | [1] Metric Scale | ||
| - | [1] Magnetic Stirrer | ||
| - | [1] Sterile Stir Bar | ||
| - | [1] P-1000 Pipet | ||
| - | [1] Sterile P-1000 Pipet Tip | ||
| - | [5] Sterile 500-milliliter Bottles | ||
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| - | 1) Retrieve 2-Liter Beaker, place on magnetic stirrer, and add stir bar. | ||
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| - | 2) Obtain 500 milliliters milliQ Water and add to beaker. | ||
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| - | 3) With clean Weigh Boat, mass out 20 grams Bacto Tryptone and add slowly to Beaker. Allow complete mixing. | ||
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| - | 4) With clean Weigh Boat, mass out 10 grams Yeast Extract and add slowly to Beaker. Allow complete mixing. | ||
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| - | 5) With clean Weigh Boat, mass out 10 grams NaCl and add to Beaker. | ||
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| - | 6) [*GLOVES*] With P-1000 Pipet set to 320 microliters, | ||
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| - | 7) While media mixes, label 5 500-milliliter Bottles with media type, date, and initials. | ||
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| - | 8) When media had completely mixed, fill Bottles with 400 milliliters of media each. | ||
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| - | 9) Loosely cap Bottles and mark with Autoclave Tape. | ||
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| - | 10) Autoclave bottles on liquid setting. | ||
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| - | 11) When autoclave cycle is complete, secure Bottle caps and store media. | ||
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