Table of Contents

Protocols

Media and Stock Solutions

Liquid Media Recipes

Making Liquid Media

Yeast Media

YEP Media and Variations

SC, SD, SD(-N), and Dropout Media

Min, Min D+, Min G+

Bacteria Media

LB and Variations

Worm Media

M9 Media

Worm S-Basic

Work S-Complete

Worm Freezing Solution

Solid Media Recipes

Making Solid Media and Pouring Plates

Yeast Plates

YEP Plates and Variations

SC, and Dropout Plates

Basic Plates

Basic +HLU Plates

GNA Plates

Bacteria Plates

LB Plates and Variations

Worm Plates

NGM Plates and Variations

Small NGM Plates

Stratalinking Plates

Stock Solutions

5mg/ml Cholesterol

50% Glucose

80% Glycerol

100mg/ml Ampicillin

1M NaOH

50% NaOH

10M NaOH

SPM +HLU

50% PEG

10x TE

1M LiOAC

0.5M LiOAC

0.1M LiOAC

50x TAE

1x TAE

0.5M EDTA

1M CaCl2

1M MgSO4

1M Potassium Phosphate pH 6.04

95% EtOH

70% EtOH

100mM FUDR (1000x)

10mM FUDR (100x)

250mM Levamisole hydrochloride

1mM Levamisole hydrochloride

2% Agarose

20mg/ml Proteinase K

100mg/ml Carbenicillin

1M IPTG

100mg/ml G418

10mg/ml EtBr

SC and Dropout Powders

Yeast Protocols

Freezing Yeast Strains

Overnight Yeast Strains

Pulling Yeast from the Freezer

Yeast Transformation (Episomal)

High Efficiency Yeast Transformation

Dual Luciferase Assay

Yeast Lifespan

Crossing Yeast Strains

Sporulation of Diploids

Tetrad Digestion and Dissection

Replica Plating

Scoring Tetrads

Yeast Colony PCR

Streaking Yeast Strains

Treating slides with ConcanavalinA

Starvation for Autophagy Imaging

Protein Extraction

Gateway Cloning

Bacterial Protocols

Freezing Bacterial Stocks

Pulling Bacterial Strains from Freezer

Making OP-50 Stock

Bacterial Transformation

Midi Prep

Mini Prep

Vacuum Manifold

Overnight Bacterial Strains

Streaking out Bacterial Strains

Bacterial PCR

Gateway Multisite LR Cloning

Worm Protocols

Seeding NGM Plates

Freezing Worm Stocks

Thawing worms Strains

Worm Lifespan

Bleaching Worms

Chunking Worms

Practicing with Worms on the Scope

Single Worm Lysis and PCR

Mounting Worms for Imaging

FUDRing Worms

Cell Culture Protocols

Cell Culture Aseptic Technique Important Notes and Points to Avoid Contamination

mouse embryonic fibroblast growth media

MEF Freeze/Thaw procedure

MEF CRISPR KO

Confirming CRISPR deletion and detecting if frameshift using Benchling

MEF QuickExtract DNA extraction procedure

MEF subculturing

MEF subculturing 24-well plate

Cellomics CX7 MEF drug exposure for ATT BIOQUEST LIVE/DEAD assay

MEF Viafect Plasmid Transfection

MEF Image Data Labeling in R

Misc. Protocols

Lab Rules to Live By

Diluting and freezing Primers

Gel Electrophoresis

Labeling a Gel

Using the pH Meter

Filter Sterilizing Stocks

Washing Lab Dishes

Autoclave Glassware

Autoclave Media

Adding Materials to the ERM

Hypoxia Chamber Setup

Refilling Ethanol Bottles

Batch Scale Bar Worm Photos Using ImageJ

Adding Drugs to Plates

Adding PEG to Plates

WAM Protocols

STLs and assembly

Annotations with LabelImg