Stratalinking Plates
Overview
When sterilizing plates, typically worm plates, other protocols have been used in our lab. If you are unsure if this protocol is appropriate for your experiment, ask someone else for help. This protocol is generally completed two days after seeding worm plates and before drugging the plates.
Materials
| UV Stratalinker or UV Crosslinker |
|---|
| ddH2O tube (if using Stratalinker) |
Procedure
1. Sterilize the inside of the Stratalinker/Crosslinker with 70% EtOH.
2. Remove and set aside the lid from a 50mL confocal tube of ddH2O tube face down on a sterilized bench.
3. Place the ddH2O tube in the center of the Stratalinker. Note: steps two and three apply only when using the Stratalinker NOT the Crosslinker.
4. Run the Stratalinker/Crosslinker once with no plates at 9999J.
5. Fill Stratalinker/Crosslinker with one layer of plates laid flat within the machine.
6. Remove the lids from every plate and set aside with the lid face down on a sterilized bench.
7. Run the Stratalinker/Crosslinker three times at 9999J for the plates.
8. Place the lids back on each plate before removing them from the Stratalinker/Crosslinker. Note: plates are often marked with a lime green stripe to indicate being stratalinked.