FUDRing Worms
Adding FUDR to worm plates with proper timing and placement should prevent progeny from developing in adult worms later in the lifepspan
Overall, 50uL of FUDR should be added to each plate (1 drop of 10 uL in lawn, and 4 drops of 10uL surrounding the lawn)
Effective FUDR timing to add FUDR after picking eggs has been identified as follows:
| Condition | N2 | Q40 |
|---|---|---|
| Vehicle | 48 hours | 64 hours |
| DMSO | 48 hours | 64 hours |
| Mupirocin | 64 hours | 80 hours |
| Borrelidin | 48 hours | |
| REP3123 | 64 hours | |
| REP8839 | 64 hours |
Note: if lay-offs are completed, these hours can vary about +/- 4 hours