— Christine Robbins 2018/10/01 16:10

1M LiOAC is used in a number of transformation protocols including episomal transformations and high efficiency transformations

Location:Shelf L

Directions Directions

• Get a beaker, and bottles and place a stir bar in the beaker
• Label all bottles and beaker with 1M LiOAc, your initials and the date
• Add MilliQ Water to about 1/4 of your final volume
• With the bar stiring, add the reagents listed below
• After all of the reagents are incorporated, fill the beaker to the final volume
• Pour the media into labeled bottles (only fill to the highest marked volume on the bottle. Example: a 500mL bottle only has gradations to 400mL, so only add 400mL media)
• With the caps partialy screwed on (you should be able to wiggle them without them coming off), label with autoclave tape
• Autoclave
• When cool, screw caps on completely and store

Media Recipe