Izabelle Chavez

Location:-20°C

Notes and Warning: Wear gloves when handling solid FUDR.

Directions

1. Wear gloves.
2. Get FUDR from -20°C dessicator.
3. Label a “100 mM FUDR” + initials + date on top and bottom a 15 mL tube.
4. Add 10 mL H2O to the tube.
5. Get a small weigh boat, small scale, and small scoopula.
6. Weigh out 0.246 g of FUDR (246 mg).
7. Dissolve FUDR in the 10 mL of H2O.
8. Vortex.
9. Filter sterilize the solution.
10. Aliquot the solution into 10 microcentrifuge tubes.
11. Label the tubes with FUDR, 100 mM, 1000x, initials, and date.
12. Place the tubes in the -20°C labeled “Worm Drug and PCR.”

Media Recipe

The directions above were made for obtaining 10 mL solution.