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10X TE

Christine Robbins 2018/10/01 15:58

Description


10X TE is used as a reagent in transformation protocols including episomal and high efficiency transformations.

Location:Shelf L

Notes and Warning: WEAR GLOVES

Media Recipe and Directions

Directions

  • In a beaker, add 1/4 the final volume of milli-Q water and a stir bar
  • Add the appropriate volume of the reagents below
  • Add milli-Q water to the final volume
  • Filter sterilize into labeled bottles

Media Recipe

Reagents Final Volume
500mL 1L
1M Tris-HCL pH=8.00 5mL 10mL
0.5M EDTA 1mL 2mL

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