Refilling Ethanol Bottles

— Ryla Cantergiani 2020/04/28 01:21

We use 70% and 95% stocks of 200-proof ethanol to sterilize our bench spaces and several pieces of equipment in lab. Squeeze bottles of 70% and 95% are on every bench, and they are refilled as part of Friday Clean-Up tasks (or when needed).

1. Collect the ethanol bottles from around the lab. Use the remaining ethanol to refill any bottles possible. Do not mix 70% and 95%. Use remaining ethanol for clean off the benches or in a protocol :) (or just dump it down the sink)
2. Fill a graduated cylinder with 350 mL of 200 proof ethanol. Do this in the fume hood. Add to squirt bottle.
3. Fill the squeeze bottle up to the line with milliQ water (about 150 mL).
4. Careful shake to mix.

1. Collect the ethanol bottles from around the lab. Use the remaining ethanol to refill any bottles possible. Do not mix 70% and 95%. Use remaining ethanol for clean off the benches or in a protocol :) (or just dump it down the sink)
2. Fill a graduated cylinder with 475 mL of 200 proof ethanol. Do this in the fume hood. Add to squirt bottle.
3. Fill the squeeze bottle up to the line with milliQ water (about 25 mL).
4. Careful shake to mix.

Ethanol is extremely flammable. Do NOT do this anywhere need an open flame.

Reagents

Equipment