Table of Contents

Pulling Bacterial Strains

— Ryla Cantergiani 2020/04/13 14:38

Overview

We have our bacterial strains frozen in the -80C freezer. When you need to use a strain, you'll have to pull it and grow it up first. The bacteria will take time to grow up, so plan ahead when organizing your experiments.

Materials

Materials
frozen bacterial strain
LB (liquid or plates)
pipette tips

Procedure

  1. Check the MM-BACTERIA-STRAINS spreadsheet to find the MM# of your bacteria
  2. Depending on what you need the bacteria for, you can grow it up on LB plates or in LB liquid media. Labels your plates/tubes.
  3. Ethanol and wipe down your bench. Turn on a flame.
  4. Using the polar bear gloves, get your bacterial strain out of the -80C freezer.
  5. Using a pipette tip (P10 or P20 work best), get a small bit of bacteria out of the tube, and gently place it onto your plate/into your liquid LB. If using a plate, let the bacteria dry before streaking out. If using liquid LB, vortex to mix.
  6. Place in the 37C incubator to grow. Plates can go in the 37 in lab. Tubes need to go in the shaking 37C in CRF.

Notes

Part Numbers