McCormick Lab Wiki

β-glucuronidase

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:21:13+00:00

ß-Glucuronidase --- Olivia Heath 2018/02/20 12:06 --- Ryla Cantergiani 2020/04/28 02:18 ---------- Description ß-Glucuronidase (or “snail juice”) is an enzyme used for digesting the ascus of a yeast tetrad during the tetrad digestion protocol. Formula: N/A [Link to MSDS] Location: 4°C Fridge UNM Barcode:

0.1m_lioac

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:03:11+00:00

0.1M LiOAc --- Christine Robbins 2018/10/01 16:54 Description ---------- 0.1M LiOAc is used in a number of transformation protocols including episomal and high efficiency transformations. Location:Shelf L Media Recipe and Directions Directions * In order to make 0.1M stock of LiOAc, dilute from the

0.5m_edta

Anonymous (anonymous@undisclosed.example.com) — 2021-04-26T11:57:57+00:00

0.5M EDTA --- Christine Robbins 2018/05/01 12:40 Description ---------- 0.5M EDTA is used to make 50x TAE and 10x TE Buffer. Location:Shelf L Media Recipe and Directions Directions * Grab a beaker of the appropriate size and add a stir bar * Add about 1/4 of the final volume of miliQ Water

0.5m_lioac

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:01:37+00:00

0.5M LiOAc --- Christine Robbins 2018/10/01 16:54 Description ---------- 0.5M LiOAc is used in a number of transformation protocols including episomal and high efficiency transformations. Location:Shelf L Media Recipe and Directions Directions * In order to make 0.5M stock of LiOAc, dilute from the

1.5_ml_flip-top_tubes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:49:04+00:00

1.5 mL Flip Top Tubes --- Ryla Cantergiani 2020/04/23 23:42 Description ---------- Flip top tubes are small, disposable plastic tubes with a small cap attached. They fit in the table-top centrifuges. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

1m_cacl2

Anonymous (anonymous@undisclosed.example.com) — 2018-11-08T09:38:05+00:00

1M CaCl2 --- Christine Robbins 2018/11/08 09:29 Description ---------- 1M CaCl2 is used in various medias for maintaining C. elegans including M9, NGM and SC medias. Location: Notes and Warning: None Media Recipe and Directions Directions * Get a beaker, and bottles and place a stir bar in the beaker

1m_lioac

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T16:32:02+00:00

1M LiOAc --- Christine Robbins 2018/10/01 16:10 Description ---------- 1M LiOAC is used in a number of transformation protocols including episomal transformations and high efficiency transformations Location:Shelf L Media Recipe and Directions Directions Directions

1m_mgso4

Anonymous (anonymous@undisclosed.example.com) — 2018-11-08T09:45:48+00:00

1M MgSO4 --- Christine Robbins 2018/11/08 09:29 Description ---------- 1M MgSO4 is used in various medias for maintaining C. elegans including M9, NGM and SC medias. Location: Notes and Warning: None Media Recipe and Directions Directions * Get a beaker, and bottles and place a stir bar in the beaker

1m_naoh

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:35:04+00:00

1M NaOH --- Christine Robbins 2018/10/01 15:02 Description ---------- 1M NaOH is used in PCR reaction for boiling yeast. Location:Shelf L Warnings WEAR GLOVES! Media Recipe and Directions Directions * Add 1/4 the final volume milli-Q water into a labeled beaker with a stir bar

1m_potassium_phosphate_ph_6.04

Anonymous (anonymous@undisclosed.example.com) — 2018-11-08T09:45:10+00:00

1M Potassium Phosphate Buffer pH=6.00 --- Christine Robbins 2018/11/08 09:40 Description ---------- 1M Potassium Phosphate Buffer pH=6.00 is used in various medias for maintaining C. elegans including M9, NGM and SC medias. Location: Notes and Warning: None Media Recipe and Directions

1mm_levamisole_hydrochloride

Anonymous (anonymous@undisclosed.example.com) — 2024-02-12T15:01:39+00:00

1mm levamisole hydrochloride Izabelle Chavez Description Location:-20°C Media Recipe and Directions Directions * Aliquot 4 µL of stock solution into one flip-top tube. * Dilute with M9 to 1000 µL (1 mL). * Store at 4°C. Note: this concentration goes bad in one week.

1x_tae

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:18:07+00:00

1X TAE * Fill Jug with 19.6 L of MilliQ Water (We generally take this from KP's lab- please ask for help if you have not done this before). NOTE: Set a timer for 10 minutes and then check it every minute. Do not let it overflow!! * Add 400 ML 50X TAE

2_agarose

Anonymous (anonymous@undisclosed.example.com) — 2024-02-12T14:22:14+00:00

2% Agarose Izabelle Chavez Description ---------- Location:drawer 46 Notes and Warning: Please indicate here if the reagent requires any special handling or storage. Media Recipe and Directions * Measure out 400 mg agarose in small weight boat with small scale.

3_8_toughspot_stickers

Anonymous (anonymous@undisclosed.example.com) — 2018-11-16T13:12:18+00:00

1/2" & 3/8" Toughspot Stickers --- Jacob Anderson 2018/11/16 13:00 Description ---------- Tough - Chemically inert, polyolefin labels resist most organic solvents and caustic agents. They adhere to most plastics†, glass, and metals without cracking, peeling, or degrading

4-nitrophenol

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:35:59+00:00

4-nitrophenol --- Ryla Cantergiani 2020/04/08 17:35 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

4_c_mini_refrigerator

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T15:33:16+00:00

4°C Mini Refrigerator --- Christine Robbins 2019/01/04 15:24 Description ---------- The 4°C Mini Refrigerator is where PCR products are stored until their gels have been ran and labeled. Yeast plates are also stored here after being thoroughly labeled and double parafilmed.

4_c_refrigerator

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T15:43:58+00:00

4°C Refrigerator --- Christine Robbins 2019/01/04 15:34 Description ---------- The 4°C Refrigerator is where a number of drugs, reagents and stock solutions are stored. This is also where bags of yeast, worm and bacteria plates are stored. Location: Notes and Warning:

5-foa_5-fluoroorotic_acid_anhydrous

Anonymous (anonymous@undisclosed.example.com) — 2019-10-15T12:56:23+00:00

5-FOA --- Olivia Heath 2018/02/07 16:24 --- Christine Robbins 2018/07/08 00:22 --- Ryla Cantergiani 2019/10/15 10:56 ---------- Description 5-FOA (5-Fluoroorotic Acid anhydrous) is used as a counter-selectable marker for +URA yeast cells. Formula: ‎C5H3FN2O4 · xH2O [Link to MSDS] Location: -20°C Freezer, in desiccator

5mg_ml_cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2018-10-30T19:04:53+00:00

5mg/ml Cholesterol --- Christine Robbins 2018/10/01 13:49 Description ---------- 5mg/ml cholesterol is used for making NGM plates for worms. Location: 4ºC Fridge Notes and Warning: This solution is dissolved in ethanol, please make sure all labels are covered in clear tape so that they do not run off. If they do start to run, re-label

6x_pcr_loading_dye

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:24:41+00:00

6X Loading Dye --- Ryla Cantergiani 2020/06/11 16:13 Description ---------- 6X PCR loading dye is used to stain your sample when run through gel electrophoresis. Dye allows you to keep track of how far your sample has moved through the gel. It also allows you to estimate that approximately the same amount of sample is added to each well. We use bromophenol blue and glycerol.

8-azaadenine

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:36:05+00:00

8-Azaadenine --- Olivia Heath 2018/02/07 14:51 --- Christine Robbins 2018/07/08 00:26 --- Ryla Cantergiani 2020/05/07 17:43 ---------- Description 8-Azaadenine is used as a positive control in the Dual Luciferase Assay protocol when using the plasmid pVW31. It causes adenine starvation to upregulate GCN4.

10m_naoh

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:36:23+00:00

10M NaOH --- Christine Robbins 2018/10/01 15:02 Description ---------- 10M NaOH is used to adjust the pH of solutions. Location:Shelf L Warnings WEAR GLOVES! Media Recipe and Directions Directions * Add 1/4 the final volume milli-Q water into a labeled beaker with a stir bar

10mm_fudr_100x

Anonymous (anonymous@undisclosed.example.com) — 2024-02-12T14:47:02+00:00

10mm fudr 100x Izabelle Chavez Description Location:4°C fridge Media Recipe and Directions Directions * Get the 100 mM 1000x stock from the -20ºC drug stock box. * Get a 15 mL tube. * Label the top and side of the tube with “FUDR,”

10x_te

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T16:10:03+00:00

10X TE --- Christine Robbins 2018/10/01 15:58 Description ---------- 10X TE is used as a reagent in transformation protocols including episomal and high efficiency transformations. Location:Shelf L Notes and Warning: WEAR GLOVES Media Recipe and Directions

15_ml_conical_tubes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:41:11+00:00

15 mL Conical Tubes --- Ryla Cantergiani 2020/04/23 23:38 Description ---------- 15 mL conical tubes have many uses in lab, including holding drug stocks, or being used in the large centrifuge. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

20_c_freezer

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T14:18:49+00:00

-20°C Freezer --- Christine Robbins 2019/01/04 14:00 Description ---------- The -20°C Freezer is where a number of reagents, PCR kits, drugs and drug stock solutions are stored as well as DNA (primers, PCR products, plasmids, salmon sperm, etc) Location: Notes and Warning:

24-well_plates_non-treated

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T15:34:13+00:00

24-Well Plates, non-treated --- Ryla Cantergiani 2020/04/23 15:33 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

30_c_incubator

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T14:41:18+00:00

30°C Incubator --- Christine Robbins 2019/01/04 14:26 Description ---------- The 30°C Incubator is where yeast samples are stored to grow. Please note, as soon as samples have grown up, they should be used and/or double parafilmed and stored in the 4°C Refrigerator or Mini Fridge.

30_c_shaker

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T15:01:39+00:00

30°C Shaker --- Christine Robbins 2019/01/04 14:49 Description ---------- The 30°C Shaker is where liquid cultures of yeast are grown up in order to be frozen, transformed, or used in the BioScreenC or Victor Nivo. Location: Notes and Warning: Do not ever turn off the incubator, only shut off the shaker when not in use. Please note whenever the shaker temperature spikes on the sheet located on the shaker.

37_c_incubator

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T15:22:31+00:00

37°C Incubator --- Christine Robbins 2019/01/04 15:03 Description ---------- The 37°C Incubator is where bacterial strains grow up on solid media. Location: Notes and Warning: Do not keep samples in this incubator for longer than 48 hours. If kept in any longer, the plates will dry out.

50_glucose

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:28:19+00:00

50% Glucose --- Christine Robbins 2018/05/01 10:40 Description ---------- The stock solution of D-glucose that we keep on hand is 50% (w/v) Glucose. This is used when making a number of yeast media recipes including, YPD liquid media, YPD plates, and all of our synthetic and drop-out media recipes.

50_ml_conical_tubes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:52:23+00:00

50 mL Conical Tubes --- Ryla Cantergiani 2020/04/23 23:50 Description ---------- 50 mL conical tubes are used for relatively small volumes of liquid. We store cholesterol, DMSO, dH2O, and other common reagents in 50 mL conical tubes for easy access. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

50_naoh

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:35:38+00:00

50% NaOH --- Christine Robbins 2018/10/01 15:02 Description ---------- 50% NaOH is used in LB liquid media. Location:Shelf L Warnings WEAR GLOVES! Media Recipe and Directions Directions * Add 1/4 the final volume milli-Q water into a labeled beaker with a stir bar

50_peg

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:39:08+00:00

50% PEG --- Christine Robbins 2018/10/01 15:22 Description ---------- 50% PEG is used in a number of transformation protocols including episomal transformation and high efficiency transformations. Location:Shelf L Warnings This is very viscous! Pipette slowly.

50x_tae

Anonymous (anonymous@undisclosed.example.com) — 2017-10-27T17:28:22+00:00

50x TAE Components(final concentrations) 2M Tris base Note, do not use Tris hydrochloride. This will result in a too acid compound 1 M acetate 100mM EDTA H2O To make 1 liter(usually kept as stock and diluted when used) 242g Tris base 57.1 mL glacial acetic acid (17.4M) N.B: GLACIAL ACETIC ACID WILL BURN YOU! USE IN HOOD AND AVOID ALL CONTACT! If in doubt about how to handle glacial acetic acid speak to your mentor first!

70_etoh

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:47:40+00:00

70% Ethanol --- Ryla Cantergiani 2020/04/28 01:43 Description ---------- 70% ethanol has a higher water content, and is therefore best for cleaning reagents that require more water to wipe up. It's good for wiping down a bench before starting a protocol. Caution should be used around fire, as 70% ethanol is still flammable.

80_c_freezer

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T14:22:37+00:00

-80°C Freezer --- Christine Robbins 2019/01/04 12:15 Description ---------- The -80°C Freezer is where all yeast (including yeast knockout collections, tet-off and damp collections), bacterial (including the Vidal and Ahringer RNAi collections), and worms strains are stored. There are also a number of kits that are kept at -80°C for long term storage (Dual Luciferase Assay Kit)

80_glycerol

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:33:22+00:00

80% Glycerol --- Christine Robbins 2018/10/01 14:19 Description ---------- The stock solution of Glycerol that we keep on hand is 80% (v/v) Glycerol. This is used when making a number of yeast media recipes including, YPG liquid media, YPG plates, and is used in freezing down yeast and bacterial cultures.

95_etoh

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:51:15+00:00

95% Ethanol --- Ryla Cantergiani 2020/04/28 01:51 Description ---------- 95% ethanol is used as a disinfectant and solvent in lab. It is HIGHLY flammable - care should be taken to keep it away from open flames. 95% ethanol is best for use to kill yeast cultures before disposal and disinfection.

96-well_plate_stickers

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:54:24+00:00

96-Well Plate Stickers --- Ryla Cantergiani 2020/04/23 23:54 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

96-well_plates_black

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:14:35+00:00

96-Well Plates, Black --- Ryla Cantergiani 2020/04/20 21:13 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

96-well_plates_non-treated

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T15:37:13+00:00

96-Well Plates --- Ryla Cantergiani 2020/04/23 15:34 Description ---------- 96-well plates are plastic plates that are used for several protocols in lab, including collecting OD600's, analysis with the Victor Nivo, etc. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

96_well_pinning_head_vp_408_multi-blot_replicator_and_accessories

Anonymous (anonymous@undisclosed.example.com) — 2019-01-25T14:46:02+00:00

96 Well Pinning Head (VP 408 Multi-Blot Replicator and accessories) --- Christine Robbins 2019/01/25 14:21 Description ---------- The 96 Well Pinning Head is used in order to duplicate the yeast and bacterial collections that are stored in the lab. Location: Cabinet 10

100mg_ml_ampicillin

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:34:25+00:00

100mg/ml Ampicillin --- Christine Robbins 2018/10/01 14:49 Description ---------- Ampicillin is used in the lab for bacteria that has a plasmid that can be selected for AMP resistance. Location: -20°C Freezer Media Recipe and Directions Directions * In a

100mm_fudr_1000x

Anonymous (anonymous@undisclosed.example.com) — 2024-02-12T14:39:35+00:00

100mm fudr 1000x Izabelle Chavez Description Location:-20°C Notes and Warning: Wear gloves when handling solid FUDR. Media Recipe and Directions Directions * Wear gloves. * Get FUDR from -20°C dessicator. * Label a “100 mM FUDR” + initials + date on top and bottom a 15 mL tube.

200_proof_ethanol

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:42:43+00:00

200 Proof Ethanol --- Christine Robbins 2018/07/08 00:17 --- Ryla Cantergiani 2020/04/28 01:38 ---------- Description Ethanol is used in the lab in a number of protocols as both a reagent and a method of sterilizing tools and work spaces. We keep stocks of 70% and 95% ethanol in squeeze bottles around lab for cleaning bench spaces. We

250mm_levamisole_hydrochloride

Anonymous (anonymous@undisclosed.example.com) — 2024-02-12T14:58:21+00:00

250mm levamisole hydrochloride Izabelle Chavez Description Location:-20°C Notes and Warning: Wear gloves when handling solid levamisole hydrochloride. Media Recipe and Directions Directions * Mix 0.12038 g (120.38 mg) of levamisole hydrochloride in 15 mL of sterile M9.

394-well_plates

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T22:22:01+00:00

394-Well Plates --- Ryla Cantergiani 2020/04/23 22:21 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

1000_μl_tips_for_vacuum_pump

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T22:19:35+00:00

1000 μL Tips for Vacuum Pump --- Ryla Cantergiani 2020/04/23 22:18 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

5415d_centrifuge

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T17:42:44+00:00

5415D Centrifuge --- Christine Robbins 2019/01/04 17:32 Description ---------- The 5415D Centrifuge is a table top centrifuge used to centrifuge samples in 1.5mL centrifuge tubes. Location: Notes and Warning: Do not use this centrifuge for high speeds on living samples. The maximum speed will generate enough heat to kill any living yeast cell. Please use the 5415R Centrifuge instead.

5415r_refrigerated_centrifuge

Anonymous (anonymous@undisclosed.example.com) — 2019-01-25T13:26:10+00:00

5415R Refrigerated Centrifuge --- Christine Robbins 2019/01/25 13:06 Description ---------- The 5415R Refrigerated Centrifuge is the refrigerated version of the 5415D tabletop centrifuge. This should be used whenever a protocol calls for spinning microcentrifuge tubes at a temperature lower than room temperature or whenever spinning live cells at high speeds.

5810r_refrigerated_centrifuge

Anonymous (anonymous@undisclosed.example.com) — 2019-01-25T14:02:34+00:00

5810R Refrigerated Centrifuge --- Christine Robbins 2019/01/25 13:26 Description ---------- The 5810R Refrigerated Centrifuge is used for spinning down samples in 50mL tubes, 15mL tubes and in microtiter plates. We currently have a fixed angle rotor with 50mL and 15mL adapters and a swinging bucket rotor that with 50mL, 15mL, and microtiter plate buckets.

acrylamide_bis_solution

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:06:49+00:00

Acrylamide/Bis Solution --- Ryla Cantergiani 2020/05/06 02:18 Description ---------- We have two solutions: 40% acrylamide/bis solution 19:1, and 30% acrylamide/bis solution 29:1. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

adding_materials_to_the_erm

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T15:46:03+00:00

Adding Materials to the ERM * Put barcode stickers on each container that needs to be entered. * Go to srs.unm.edu * Click ERM 9 * Ask Christine to log in * Click Material search -> Create container * Type the proper barcode * Click add, then continue.

adding_things_to_the_erm

Anonymous (anonymous@undisclosed.example.com) — 2019-05-08T15:40:13+00:00

Adding Things to the ERM Procedure ---------- Go to The ERM Website and log in. 1. Click the icon for materials search. ---------- 2. Search for the name of the chemical you are looking for and press enter. Press the create container button. 3. Type the bar code of the sticker that you attached to the chemical container. If there are multiple bar codes, separate them by commas.

adenine_7h-purin-6-amine

Anonymous (anonymous@undisclosed.example.com) — 2019-10-17T11:07:08+00:00

Adenine --- Christine Robbins 2018/01/19 12:54 --- Ryla Cantergiani 2019/10/17 9:05 ---------- Description Adenine (7H-Purin-6-amine) Formula: C5H5N5 [Link to MSDS] Location: WW-Chemical Shelf, in “Amino Acid Powders A-G” UNM Barcode: 117023, 117067, 117068, 117069 Notes and Warning:

adhesive_foil

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:31:11+00:00

Adhesive Foil --- Ryla Cantergiani 2020/04/20 21:29 Description ---------- Adhesive foil is foil that has an adhesive on one side to create a seal. We use adhesive foil on the frozen-down YKO-collection yeast plates to cover the wells. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

administrative_minutiae

Anonymous (anonymous@undisclosed.example.com) — 2019-05-08T15:17:35+00:00

Lab Meeting Protocol Ordering Primers Adding Things to the ERM

agar_bacteriological

Anonymous (anonymous@undisclosed.example.com) — 2019-10-17T11:18:21+00:00

Agar, Bacteriological --- Christine Robbins 2018/01/19 13:02 --- Ryla Cantergiani 2019/10/17 9:17 ---------- Description Agar Formula: C14H24O9 [Link to MSDS] Location: XX-Chemical Shelf UNM Barcode: 116992 Notes and Warning: No applicable hazard/precautionary statements. Order Information

agarose

Anonymous (anonymous@undisclosed.example.com) — 2019-10-22T12:42:23+00:00

Agarose --- Christine Robbins 2018/01/19 13:08 --- Ryla Cantergiani 2019/10/22 12:41 ---------- Description Agarose Formula: C24H38O19 [Link to MSDS] This link is to Genesee support, there is no MSDS on their website. Location: XX-Chemical Shelf UNM Barcode: 116991 Notes and Warning:

ammonium_sulfate

Anonymous (anonymous@undisclosed.example.com) — 2019-10-17T14:41:34+00:00

Ammonium Sulfate --- Christine Robbins 2018/01/19 13:13 --- Ryla Cantergiani 2019/10/17 12:39 ---------- Description Ammonium Sulfate Formula: H8N2O4S [Link to MSDS] Location: XX-Chemical Shelf UNM Barcode: 116993 Notes and Warning: Store at room temperature. Keep away from strong bases and strong oxidizing agents.

amphotericin_b

Anonymous (anonymous@undisclosed.example.com) — 2019-10-22T12:51:56+00:00

Amphotericin B --- Olivia Heath 2018/02/20 13:30 --- Ryla Cantergiani 2019/10/22 12:51 ---------- Description Amphotericin B Antibiotic, generally used in liquid worm culture assays. Formula: C47H73NO17 [Link to MSDS] Location: -20°C Freezer UNM Barcode: 117102 Notes and Warning:

ampicillin

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:40:09+00:00

Ampicillin --- Olivia Heath 2018/02/07 14:56 ---------- Description Ampicillin Antibiotic, used as a selection marker in bacteria. We keep a 100 mg/mL solution in the -20°C freezer. Link to Overnighting Bacterial Strains Formula: ‎C16H18N3NaO4S [Link to MSDS] Location: 4°C Fridge in dissector; aliquots kept in -20°C

annotations_with_labelimg

Anonymous (anonymous@undisclosed.example.com) — 2026-05-14T17:12:29+00:00

Setup Guide for Frame Annotation Syncthing and LabelImg [--- Alex Achusim 2026/05/14 17:09] Overview ---------- Enter description of the media, please link any protocols that it is used in. In this section you put why we do this protocol and how it works very generally,as well as what the protocol(s) immediately preceding this and immediately following this are (with wiki links), if applicable.

apex_dna_ladder_i

Anonymous (anonymous@undisclosed.example.com) — 2019-10-22T14:18:59+00:00

Apex PCR DNA Ladder I --- Olivia Heath 2018/02/20 12:55 --- Ryla Cantergiani 2019/10/22 14:17 ---------- Description Apex PCR DNA Ladder I 200-12,000bp Formula: N/A [Link to MSDS] Location: -20°C Fridge UNM Barcode: 117089 Notes and Warning: Order Information ---------- Vendor:

apex_pcr_dna_ladder

Anonymous (anonymous@undisclosed.example.com) — 2019-10-22T14:28:40+00:00

Apex PCR DNA Ladder --- Olivia Heath 2018/02/20 12:55 --- Ryla Cantergiani 2019/10/22 14:28 ---------- Description Apex PCR DNA Ladder 100-3,000bp Formula: N/A Location: -20°C Fridge UNM Barcode: 117088 Notes and Warning: Order Information ---------- Vendor: Genesee

autoclave

Anonymous (anonymous@undisclosed.example.com) — 2020-04-08T20:13:21+00:00

Autoclave --- Ryla Cantergiani 2020/04/08 19:13 Description ---------- Autoclaves use high temperature and pressure to sterilize equipment and reagents. There are three autoclaves that we use. The small autoclave is in our lab. It can fit one 2 L flask, or about three 1 L bottles. Do not overfill. The other two autoclaves are in CRF on the first and second floors.

autoclave_glassware

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T17:21:39+00:00

Autoclaving Glassware * Before you are ready to autoclave the glassware, you need to get it prepped. To do this you will make sure your glassware is dry, add aluminum foil to cover the opening, and then add autoclave tape to the foil. * Once everything has foil and tape, you will bring your cart full of glassware to one of the two autoclaves.

autoclave_media

Anonymous (anonymous@undisclosed.example.com) — 2018-08-14T17:14:08+00:00

Media Autoclave Protocol 1) Cover media container in aluminum foil (left of glassware sink). 2) Mark foil cover with green-and-white-striped autoclave tape (found on cart). 3) Place media container in white tub on autoclave cart. 4) Ensure that a pitcher and a set of orange

bacterial_pcr

Anonymous (anonymous@undisclosed.example.com) — 2025-08-12T18:10:33+00:00

Bacterial PCR --- Madison Otero 2025/08/12 17:29 Overview ---------- PCR protocol for testing bacteria for RNAi inserts. Materials ---------- * Baxter water * 2X bacteria master mix * bacteria in liquid media * PCR primers * ice * flip top tubes

bacterial_transformation

Anonymous (anonymous@undisclosed.example.com) — 2018-11-19T18:05:09+00:00

Bacterial Transformations --- Jacob Anderson 2018/11/16 13:29 Overview ---------- This protocol is adapted from Kristan Steffen, (UW). This protocol is meant to make more plasmids! Through exogenous uptake we can introduce new constructs to bacteria, force their machinery to make it copies of the desired plasmid, and harvest the product. The use of chemically competent E.coli cells allow for easy transformations while maintaining the plasmid without rearrangement of the plasmid DNA.

bacto_peptone

Anonymous (anonymous@undisclosed.example.com) — 2019-10-22T14:35:58+00:00

Bacto Peptone Christine Robbins 2018/01/19 13:30 --- Ryla Cantergiani 2019/10/22 14:29 ---------- Description Bacto Peptone Used in making media NGM Plates YEP and Variations Formula: [Link to MSDS] Location: YY-Chemical Shelf UNM Barcode: 117072, 117073 Notes and Warning: USE THIS PEPTONE FOR NGM PLATES Order Information

batch_scale_bar_worm_photos_using_imagej

Anonymous (anonymous@undisclosed.example.com) — 2021-02-10T16:54:05+00:00

Batch Scale Bar Worm Photos Using ImageJ --- Sean Delica 2021/02/10 14:48 Procedure ---------- Note: Magnification of the wormscope needs to be calibrated using a micrometer, if that has not been done yet. * Go to amscope application, which has the data for your magnification specs.

baxter_water

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:15:00+00:00

Baxter Water --- Ryla Cantergiani 2020/06/09 16:14 Description ---------- Our Sterile Water source is Baxter “Sterile Water for Irrigation, USP”, which we've also found to be RNAse and DNAse free. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

beakers

Anonymous (anonymous@undisclosed.example.com) — 2019-01-25T16:09:23+00:00

Beakers --- Christine Robbins 2019/01/25 15:09 Description ---------- Beakers are used in the lab to make and hold various liquid medias. Location:Shelves B C E F Equipment maintenance: Please throw away and replace any chipped or cracked glassware. Order Information

berberine_chloride

Anonymous (anonymous@undisclosed.example.com) — 2019-10-31T12:56:35+00:00

Berberine Chloride --- Christine Robbins 2018/01/19 13:35 --- Ryla Cantergiani 2019/10/31 10:56 ---------- Description Berberine Chloride Formula: C20H18ClNO4 [Link to MSDS] Location: XX-Chemical Shelf UNM Barcode: 116994 Notes and Warning: Order Information ---------- Vendor: TCI

bioscreen_plates

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T15:30:53+00:00

Bioscreen Plates --- Ryla Cantergiani 2020/04/23 15:27 Description ---------- Bioscreen plates are 100-well honeycomb patterned plates meant for use in the bioscreen. These may be used for growth assays, etc. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

bioscreenc

Anonymous (anonymous@undisclosed.example.com) — 2020-04-08T19:10:19+00:00

Bioscreen C --- Ryla Cantergiani 2020/04/08 18:37 Description ---------- We have access to a Bioscreen C Automated Growth Curve System, which automagically measures the growth curves in all wells of 2 100 well plates while shaking and incubating them. It can be used for anything you can grow in 150ul liquid cultures; we use it for yeast.

bleaching_worms

Anonymous (anonymous@undisclosed.example.com) — 2023-06-21T13:57:06+00:00

There are two bleaching methods for worms. Worms can be bleached to remove contamination from a strain or to obtain a large quantity of eggs. Below a protocol for both methods can be found. BLEACHING EGGS FOR STERILIZATION Worm Bleach Recipe 10 mL Bleach 4 mL 10 M NaOH 26 mL Distilled Water

borrelidin

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:46:25+00:00

Borrelidin --- Ryla Cantergiani 2020/06/08 21:44 Description ---------- Borrelidin is an antiobiotic. We keep 100mM solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

bottles

Anonymous (anonymous@undisclosed.example.com) — 2019-01-25T18:09:10+00:00

Bottles --- Christine Robbins 2019/01/25 16:14 Description ---------- Bottles are where liquid media and reagents are stored. Location:Shelves B and C Equipment maintenance: Please throw away and replace any bottles with chipped or cracked glass. Order Information

bovine_serum_albumin

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:05:38+00:00

Bovine Serum Albumin --- Ryla Cantergiani 2020/05/06 02:10 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

bromophenol_blue

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T15:59:50+00:00

Bromophenol Blue --- Christine Robbins 2018/01/19 13:39 ---------- Description Bromophenol Blue Formula: C19H10Br4O5S [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 116995 Notes and Warning: Order Information ---------- Vendor: Amazon Catalog Number: GRM914-5G

c-his_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:06:59+00:00

C-his Dropout Powder --- Ryla Cantergiani 2020/05/07 19:05 Description ---------- C-his dropout powder contains all the essential amino acids except histidine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-leu_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:12:35+00:00

C-Leu Dropout Powder --- Ryla Cantergiani 2020/05/07 19:12 Description ---------- C-leu dropout powder contains all the essential amino acids except leucine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-lys_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:11:18+00:00

C-Lys Dropout Powder --- Ryla Cantergiani 2020/05/07 19:10 Description ---------- C-lys dropout powder contains all the essential amino acids except lysine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-met_cys_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:17:30+00:00

C-Met Cys Dropout Powder --- Ryla Cantergiani 2020/05/07 19:14 Description ---------- C-met cys dropout powder contains all the essential amino acids except methionine and cysteine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-met_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:14:41+00:00

C-Met Dropout Powder --- Ryla Cantergiani 2020/05/07 19:13 Description ---------- C-met dropout powder contains all the essential amino acids except methionine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-met_thr_cys_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:10:09+00:00

C-met thr cys Dropout Powder --- Ryla Cantergiani 2020/05/07 19:07 Description ---------- C-met thr cys dropout powder contains all the essential amino acids except methionine, threonine, and cysteine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-met_thr_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:16:52+00:00

C-Met Thr Dropout Powder --- Ryla Cantergiani 2020/05/07 19:15 Description ---------- C-met thr dropout powder contains all the essential amino acids except methionine and threonine. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

c-trp_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T19:16:05+00:00

C-Trp Dropout Powder --- Ryla Cantergiani 2020/05/07 19:15 Description ---------- C-trp dropout powder contains all the essential amino acids except tryptophan. This media is often used for selectivity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

calcium_chloride

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:28:30+00:00

Calcium Chloride --- Christine Robbins 2018/01/19 13:44 ---------- Description Calcium Chloride. We keep a pre-made 1M stock on Shelf R. Formula: CaCl2 [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 116996 Notes and Warning: Order Information ---------- Vendor:

carbenicillin_disodium_salt

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:37:57+00:00

Carbenicillin Disodium Salt --- Olivia Heath 2018/02/07 15:12 ---------- Description Carbenicillin Disodium Salt We keep a 100 mg/mL solution in the -20°C freezer. Formula: ‎C17H16N2Na2O6S [Link to MSDS] Location: 4°C Fridge UNM Barcode: 117086 Notes and Warning:

cdp-star

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:06:18+00:00

CDP-Star --- Ryla Cantergiani 2020/05/06 02:15 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

cell_counter

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T12:39:59+00:00

Cell Counter --- Christine Robbins 2019/02/05 12:31 Description ---------- The cell counter is generally used in the lab in order to perform thrashing and pumping assays with C. elegans. Location:Drawer 17 Order Information ---------- Item Vendor

cell_culture_aseptic_technique_important_notes_and_points_to_avoid_contamination

Anonymous (anonymous@undisclosed.example.com) — 2021-03-29T11:55:28+00:00

Overview The purpose of this page is to provide basic points of the aseptic technique required when doing cell culture and handling all cell culture reagents and materials. Contamination is common with beginners. And every month I hear of yeast infections, bacterial contamination, or something of the sort from the cell culture room.

cellomics_cx7_mef_drug_exposure_for_att_bioquest_live_dead_assay

Anonymous (anonymous@undisclosed.example.com) — 2021-03-22T15:38:16+00:00

Overview This protocol will outline how to prepare your cells for fluorescent microscopy analysis with the CX7 located in the AIM core using a LIVE/DEAD assay supplied from ATT Bioquest. This protocol will also outline how to exposure your adherent cell lines to drug concentrations without worrying about media evaporation.

chloramphenicol

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T15:18:15+00:00

Chloramphenicol --- Olivia Heath 2018/02/07 15:16 ---------- Description Chloramphenicol Formula: ‎C11H12Cl2N2O5 [Link to MSDS] Location: 4°C Fridge UNM Barcode: 117087 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: 97061-244

chloroform

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:00:48+00:00

Chloroform --- Olivia Heath 2018/02/07 14:06 ---------- Description Chloroform Formula: ‎CHCl3 [Link to MSDS] Location: Cabinet 8 - Under Hood UNM Barcode: 117075 Notes and Warning: Undergraduates are NOT allowed to handle chloroform. If it is needed in a protocol, please ask your friendly neighborhood lab tech or graduate student for help. No one wants to fill out the paperwork of y'all getting chloroformed.

cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:32:17+00:00

Cholesterol --- Christine Robbins 2018/01/19 13:48 ---------- Description Cholesterol. We keep a pre-made 5mg/mL solution in the 4°C refrigerator. We use the 5mg/mL solution in making NGM. Formula: C27H46O [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 116997 Notes and Warning:

chunking_worms

Anonymous (anonymous@undisclosed.example.com) — 2018-05-04T14:59:02+00:00

Chunking Worms YOU WILL NEED: * 200 proof ethanol * FIRE * Xacto knife * Plate(s) that you are chunking from * Plate(s) that you are chunking to As with all procedures when you are working with live things, please clean off the bench. *

citric_acid_monohydrate

Anonymous (anonymous@undisclosed.example.com) — 2020-04-08T18:07:41+00:00

Citric Acid Monohydrate --- Ryla Cantergiani 2020/04/08 17:41 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

clear_nail_polish

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:34:14+00:00

Clear Nail Polish --- Ryla Cantergiani 2020/04/20 22:32 Description ---------- We use clear nail polish when making microscope slides to seal the cover slip to the slide. This prevents the coverslip from moving around, especially when using a 100x oil objective.

concanavalin_a

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:32:43+00:00

Concanavalin A --- Ryla Cantergiani 2020/06/08 21:12 Description ---------- Concanavalin A (ConA) is a lectin. We use ConA when making microscope slides to prevent the cells from moving around. We have both 5 mg/mL and 0.5 mg/mL solutions in the -20°C freezer.

confirming_crispr_deletion_and_detecting_if_frameshift_using_benchling

Anonymous (anonymous@undisclosed.example.com) — 2021-03-26T17:56:44+00:00

Overview The purpose of this protocol is to take the sequencing data that was retrieved from eurofins and aligning the sequences to get a consensus sequence and then aligning that consensus sequence with the wildtype to confirm the knockout and seeing if you could predict a frameshift.

coomassie_plus_protein_assay_reagent

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:33:28+00:00

Coomassie Plus Protein Assay Reagent --- Ryla Cantergiani 2020/04/28 02:30 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

copper_ii_sulfate_pentahyrate

Anonymous (anonymous@undisclosed.example.com) — 2020-04-08T17:43:04+00:00

Copper (II) Sulfate Pentahydrate --- Ryla Cantergiani 2020/04/08 17:41 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

crossing_yeast_strains

Anonymous (anonymous@undisclosed.example.com) — 2018-06-26T19:15:51+00:00

Crossing Yeast Strains 1) Clean a benchtop with 70% ethanol 2) Label appropriate number of plates as shown below: 3) Turn on flame. Using a sterile tip, gently spread the appropriate strain on the correct line. Do not allow any lines to touch.

culture_tubes_and_caps

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T12:52:21+00:00

Culture Tubes and Caps --- Christine Robbins 2019/02/05 12:40 Description ---------- Culture tubes are used in order to grow liquid cultures of yeast and bacteria. Location:Shelves UU and VV Notes and Warning: Dispose of any tubes that are broken, cracked or chipped.

cycloheximide

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:09:16+00:00

Cycloheximide --- Ryla Cantergiani 2020/05/07 17:40 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. We have a 10 mg/mL and 20 mg/mL solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

d-_-raffinose_pentahydrate

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:02:50+00:00

D-(+)-Raffinose Pentahydrate --- Christine Robbins 2018/01/19 13:51 ---------- Description D-(+)-Raffinose Pentahydrate Formula: C18H32O16 5H2O [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 116998 Notes and Warning: Order Information ---------- Vendor: Amazon / Chemsavers

d-glucose

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:26:43+00:00

D-Glucose --- Christine Robbins 2018/01/19 13:59 ---------- Description D-Glucose. We keep a pre-made stock of 50% Glucose on shelf R. Formula: C6H12O6 [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 117000 Notes and Warning: Order Information ---------- Vendor:

dapi

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:06:35+00:00

DAPI --- Ryla Cantergiani 2020/05/06 02:16 Description ---------- DAPI is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.

depc_diethyl_dicarbonate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:29:01+00:00

DEPC (diethyl dicarbonate) --- Ryla Cantergiani 2020/05/06 02:12 Description ---------- DEPC is used to inactivate RNase enzymes in water and laboratory equipment. We keep DEPC water on Shelf DD. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

di-sodium_hydrogen_phosphate_anhydrous

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:04:39+00:00

Di-Sodium hydrogen phosphate anhydrous --- Christine Robbins 2018/01/19 14:03 ---------- Description Di-Sodium hydrogen phosphate anhydrous Formula: Na2HPO4 [Link to MSDS] Location: LL-Chemical Shelf UNM Barcode: 116999 Notes and Warning: Order Information ----------

dig_blocking_solution

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:33:59+00:00

Dig Blocking Solution --- Ryla Cantergiani 2020/06/11 16:33 Description ---------- We have a 10X Dig blocking solution. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

dig_detection_buffers

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:28:15+00:00

Dig Detection Buffer --- Ryla Cantergiani 2020/06/11 15:26 Description ---------- Dig detection buffer is used in immunological detection. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

dig_wash_buffer

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:29:16+00:00

Dig Wash Buffer --- Ryla Cantergiani 2020/06/11 15:28 Description ---------- Dig wash buffer is used in immunological detection. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

diluting_and_freezing_primers

Anonymous (anonymous@undisclosed.example.com) — 2018-06-29T15:04:09+00:00

Diluting and Freezing Primers * Grab the primer binder located on the computer desk and open it up. * Open up the Dropbox folder on the computer and proceed to the folder named “mccormicklabshared”. * Within the folder locate the MM-Primer sheet and open it. It should be showing as soon as you open up the

dmso-safe_syringe_filter

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T20:56:31+00:00

DMSO Safe Syringe Filters --- Ryla Cantergiani 2020/04/20 20:54 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

dmso

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:05:22+00:00

DMSO --- Olivia Heath 2018/02/07 14:23 ---------- Description DMSO Formula: ‎C2H6OS [Link to MSDS] Location: 59- Dark Chemical UNM Barcode: 117047 Notes and Warning: Order Information ---------- Vendor: Ebay / DMSO Store Catalog Number: 1000082 Order Link:

dntp_mix_10um_each

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T13:08:50+00:00

dNTP mix 10µM each * -- Olivia Heath 2018/02/20 13:05 ---------- Description dNTP mix 10µM each Formula: N/A Location: -20°C Freezer UNM Barcode: 117104 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: PAU1515 Order Link

doxycycline

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:09:27+00:00

Doxycycline --- Ryla Cantergiani 2020/05/06 01:53 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. We have 50 mg/mL solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

dtt_1_4-dithio-dl-threitol

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:07:36+00:00

DTT (1,4-Dithio-DL-threitol) --- Ryla Cantergiani 2020/05/07 17:32 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

dual_luciferase_assay

Anonymous (anonymous@undisclosed.example.com) — 2018-06-26T13:14:31+00:00

DUAL LUCIFERASE ASSAY Overnight Phase: 1) Retrieve 4 overnight tubes per strain (shelves right of microwave), 5mL disposable pipets (black rack left of monitor row), and Pipet Aide (throughout workstations). 2) Label 4 tubes per strain (MM####, genotype, mating type, initials, date). Number tubes for each strain 1-4.

dualluciferase_reporter_assay

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T12:34:01+00:00

DualLuciferase Reporter Assay --- Olivia Heath 2018/02/20 12:32 ---------- Description Formula: N/A Location: -80°C Freezer UNM Barcode: 117105 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: PAE1910 Order Link

edta

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:24:24+00:00

EDTA --- Christine Robbins 2018/01/19 14:19 ---------- Description EDTA. We keep a pre-made 0.5M solution on Shelf O. Formula: C10H12O8CaN2Na2·2H2O or C10H16N2O8 or ((HOOCCH2)2NCH2)2 [Link to MSDS] Location: LL - Chemical Shelf UNM Barcode: 117001 Notes and Warning: Order Information

equipment

Anonymous (anonymous@undisclosed.example.com) — 2020-04-13T14:51:07+00:00

McCormick Lab Equipment Here you will find all equipment that the McCormick Lab has or uses. Lab Members: If we receive new equipment or you notice that something we have does not have a wiki page, please stop what you are doing and create one using the

ethidium_bromide

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:31:15+00:00

Ethidium Bromide --- Christine Robbins 2018/01/19 14:32 ---------- Description Ethidium Bromide. We keep a pre-made 10mg/mL solution that we use to stain gels on Shelf BBB. Formula: C21H20BrN3 [Link to MSDS] Location: 59 - Dark Chemicals UNM Barcode: 117048 Notes and Warning:

filling_out_your_timesheet

Anonymous (anonymous@undisclosed.example.com) — 2019-09-17T13:31:45+00:00

You should receive a timesheet template from the BMB Department. 1. Fill out your UNM ID, name, and the pay period. This is in the top row of your timesheet. 2. Fill out your in and out times for that period. These are the green boxes. Make sure that the In/Out Calc box shows the correct total value for that day.

filter_sterilizing_stocks

Anonymous (anonymous@undisclosed.example.com) — 2018-05-01T11:55:00+00:00

Filter Sterilizing Stock Solutions --- Christine Robbins 2018/05/01 11:05 Description Often, stock solutions of reagents and drugs cannot be sterilized by being autoclaved. Drugs in particular will degrade at high temperatures. Below are directions for filter sterilizing solutions based on their volume.

flasks

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T13:07:15+00:00

Flasks --- Christine Robbins 2019/02/05 12:53 Description ---------- Flasks are used for making and sterilizing solid media that will be poured into plates. Location:Shelves A, D, E and F Equipment maintenance: Please throw away and replace any chipped or cracked glassware.

formamide

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:28:33+00:00

Formamide --- Ryla Cantergiani 2020/04/28 02:27 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

freezing_bacterial_stocks

Anonymous (anonymous@undisclosed.example.com) — 2020-04-13T16:08:58+00:00

Freezing Bacterial Strains --- Ryla Cantergiani 2020/04/10 23:49 Overview ---------- Bacteria can be frozen at -80C, and that stock can be taken at a later date to re-grow up the strain. We freeze down strains that we've created or ordered. A full list of strains can be found in the MM-BACTERIA-STRAINS spreadsheet in the dropbox.

freezing_worm_stocks

Anonymous (anonymous@undisclosed.example.com) — 2018-07-12T17:03:09+00:00

Freezing Worm Strains * Pick 20 young adult worms onto 10 cm plates that were seeded with 1 mL OP50. If you are working with two plates, then you should have 40 worms in total. If the worms are growing at a temperature of 15 C they will be ready to freeze within 1 week, however, if they are growing at a temperature of 20 C they will be ready in the timespan of 4 days.

freezing_yeast_strains

Anonymous (anonymous@undisclosed.example.com) — 2018-10-31T17:17:13+00:00

Freezing Yeast Strains --- Olivia Heath 2018/05/03 17:28 Overview ---------- When we obtain or create a new yeast strain, the strain should be IMMEDIATELY frozen down and added to the collection of strains in the -80°C Freezer. AT THE SAME TIME, the complete strain information must be added to the lab yeast strains spreadsheet, MM-Yeast-Strains.ods, which is in the

fudr

Anonymous (anonymous@undisclosed.example.com) — 2023-05-22T16:28:04+00:00

FUDR --- Olivia Heath 2018/02/20 13:01 ---------- Description We use FUDR in worm lifespans. FUDR is a DNA synthesis inhibitor. When given to L4/young adult worms, it will prevent them from laying eggs that will hatch. We use 10x FUDR in lifespan experiments. FUDR has been shown to increase explosions in lifespans.

fudring_worms

Anonymous (anonymous@undisclosed.example.com) — 2024-12-02T16:31:58+00:00

FUDRing Worms Adding FUDR to worm plates with proper timing and placement should prevent progeny from developing in adult worms later in the lifepspan Overall, 50uL of FUDR should be added to each plate (1 drop of 10 uL in lawn, and 4 drops of 10uL surrounding the lawn)

fume_hood

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T13:16:46+00:00

Fume Hood --- Christine Robbins 2019/02/05 13:08 Description ---------- The fume hood is used for storing all acidic, basic or flammable chemicals. The vacuum pump set up is also located in the hood as well as all hypoxia related work. Please be sure to leave all chemicals in the hood while using them to avoid inhaling any fumes.

g418_sulfate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:09:55+00:00

G418 Sulfate --- Olivia Heath 2018/02/07 15:20 --- Ryla Cantergiani 2020/05/06 02:05 ---------- Description G418 sulfate is an antibiotic which inhibits translation by binding irreversibly to the ribosome. It is used in yeast to select for the presence of the KanMX marker. KanMX encodes an enzyme which renders the G418 molecule inactive.

gateway_cloning

Anonymous (anonymous@undisclosed.example.com) — 2020-11-16T20:56:53+00:00

Gateway Cloning [Insert Signature here. You must be signed in to do this] Overview ---------- Gateway cloning is used to insert genes into a plasmid for expression. In our lab we use the Invitrogen pENTR TOPO Cloning Kit for Gateway cloning reactions.

gateway_multisite_lr_cloning

Anonymous (anonymous@undisclosed.example.com) — 2018-11-21T19:43:33+00:00

Gateway Multisite Cloning LR Clonase --- Jacob Anderson 2018/11/21 12:05 Overview Guidelines and instructions are provided to perform a MultiSite Gateway® Pro LR recombination reaction between suitable supercoiled entry clones and a supercoiled destination vector using LR Clonase™ II Plus enzyme mix. We recommend including and negative control (no LR Clonase™ II Plus) and one or more positive control reactions.

gc10_competent_cells_e._coli

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T12:38:22+00:00

GC10 Competent Cells 10 x 50ul/Unit * -- Olivia Heath 2018/02/20 12:34 ---------- Description GC10 Competent Cells 10 x 50ul/Unit Formula: N/A Location: -80°C Freezer UNM Barcode: N/A Notes and Warning: Order Information ---------- Vendor: Genesee

gel-loading_tips

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T15:25:56+00:00

Gel-Loading Tips --- Ryla Cantergiani 2020/04/23 15:24 Description ---------- Gel-loading tips are disposable pipette tips that are easier for loading the wells of a gel. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

gel_electrophoresis

Anonymous (anonymous@undisclosed.example.com) — 2022-02-15T18:07:24+00:00

Gel Electrophoresis --- Olivia Heath 2018/05/01 16:28 Protocol ---------- Setting up a Gel Decide how many wells you are going to need. We have 12, 20, and 25 well combs. Keep in mind that you will need to put HL on either side of your samples. You will also need to make your asymmetrical so as to avoid confusion. If your gel is symmetrical, you will need to grab the XACTO knife and cut off the top right corner.

gel_electrophoresis_apparatus

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T13:37:42+00:00

Gel Electrophoresis Apparatus --- Christine Robbins 2019/02/05 13:20 Description ---------- The Gel Electrophoresis Apparatus are used in order to cast and run aggarose gels to separate out DNA fragments based on size. We have 2 sizes of gel boxes, large (drawer 65) and small (drawer 63). Large boxes have combs for 12, 20 and 25 wells and should be run at 160V or lower. Small boxes have combs for 12 wells and should be run at 100V or lower.

gel_electrophoresis_power_source

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T14:08:19+00:00

Gel Electrophoresis Power Source --- Christine Robbins 2019/02/05 13:56 Description ---------- The Gel Electrophoresis Power Source is used on order to power the gel boxes used in order to run DNA aggarose gels. Location:Shelf YY [Equipment Manual] Order Information ---------- Item

gelatin

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:08:25+00:00

Gelatin --- Christine Robbins 2018/01/23 12:12 Description ---------- Formula: C6H12O6 [Link to MSDS] Location:Shelf LL UNM Barcode: 117002 Notes and Warning: None Order Information ---------- Vendor: VWR / Aldon Part Number: 470301-134 / GG0025-500GP Order Link:

glacial_acetic_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:14:57+00:00

Glacial Acetic Acid --- Olivia Heath 2018/01/24 13:22 --- Ryla Cantergiani 2020/04/28 01:17 ---------- Description We use acetic acid when preparing out Tris-buffer, Acetic Acid, EDTA (TAE) stock. Formula: CH3COOH [Link to MSDS] Location: 7- under Hood UNM Barcode: 117007 Notes and Warning:

glass_hockey_stick

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T14:27:56+00:00

Glass Hockey Stick (Cell Spreader) --- Christine Robbins 2019/02/05 14:10 Description ---------- Glass Hockey Stick or Cell Spreaders are used in order to spread cultures of yeast or bacteria onto plates. They are used for seeding NGM plates with OP-50 and in transformations for spreading cells onto selective media. These hokey sticks are made by melting straight glass rods in two places to form the shape seen below.

glass_rods

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:39:41+00:00

Glass Rods --- Ryla Cantergiani 2020/04/20 22:37 Description ---------- We use glass rods to make the hockey sticks for seeding large plates, etc. Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

gloves

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:40:12+00:00

Gloves --- Ryla Cantergiani 2020/04/20 21:32 Description ---------- Some protocols, such as those that use corrosive chemicals or RNAse-free protocols, may require you to wear gloves. we use powder-free nitrile gloves. These are single use gloves. If you use the last pair of a box of gloves, please replace the box. If there is not a box of your size open, grab a box of your size and keep it with the rest of the open boxes.

glycerol

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:26:52+00:00

Glycerol --- Christine Robbins 2018/01/23 12:20 Description ---------- Glycerol is used to freeze down samples of yeast, bacteria and worms. It is also used as a carbon source in yeast media (see YPG). We keep a pre-made stock of 80% Glycerol on Shelf R. Formula:

glycine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:10:21+00:00

Glycine --- Olivia Heath 2018/01/24 13:44 ---------- Description Glycine Formula: C2H5NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117024 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number: B00EOXU0MM

glycolic_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:19:40+00:00

Glycolic Acid * -- Olivia Heath 2018/01/24 13:54 ---------- Description Glycolic Acid Formula: C2H4O3 [Link to MSDS] Location: Cabinet 7- under Hood UNM Barcode: 117081, 117080 Notes and Warning: Order Information ---------- Vendor: Fisher Scientific Catalog Number:

gna_plates

Anonymous (anonymous@undisclosed.example.com) — 2018-07-02T14:47:43+00:00

Making GNA Plates * First, grab a 250 mL flask and a 100 mL graduated cylinder from the glassware shelves (Shelf E and Shelf F). Make sure they have foil covering them and that the autoclave tape that is there is striped black. * Gather a bag of scoopulas, a mass scale, and 4 medium-sized weighboats and place next to the hot plates on the bench.

grad_student

Anonymous (anonymous@undisclosed.example.com) — 2019-10-09T14:36:14+00:00

NSF GRFP NIAID NIH F3x S-CAP Travel Award BSGP travel award Forms at the bottom of the page GPSA travel awards

graduate_student_general_information

Anonymous (anonymous@undisclosed.example.com) — 2018-12-10T13:28:46+00:00

Submitting Research Grades at the end of semester Selectives and Concentrations Qualifying Exam

graduated_cylinders

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T15:02:16+00:00

Graduated Cylinders --- Christine Robbins 2019/02/05 14:31 Description ---------- Graduated Cylinders are used in order to precisely measure liquids. Location:Shelves D and E Order Information ---------- Item Vendor

green_tea_an_aid_to_weight_loss

Anonymous (anonymous@undisclosed.example.com) — 2019-06-18T03:22:20+00:00

Green Tea, An Aid To Weight Loss Auresoil Sensi & Secure преглед Are you've wondering ideal way to convert extremely guy friend to the man you're dating? Don't you wish that might just stop treating you like one from the boys and see you whilst the lady you may be? How do you take that step from finding yourself in a platonic relationship to some romantic some? Before you go ahead and concentrate on getting your best guy friend into your boyfriend, you need to that you're treading best point o…

hazardous_waste_pick_up

Anonymous (anonymous@undisclosed.example.com) — 2021-05-20T21:10:19+00:00

Hazardous Waste Pick Up UNM risk safety services has an outside vendor that pick up hazardous waste about once a week on Fridays. If you need any waste picked up, follow the steps below in order to make sure things are done properly. In general, the only waste we create that is hazardous is

heat_block

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T15:17:49+00:00

Heat Block --- Christine Robbins 2019/02/05 15:04 Description ---------- The lab has 2 heat blocks that are kept at 2 different temperatures. One is at 42°C and is used during transformations for the heat shock step. The other is set to 95°C and is used to boil salmon sperm during transformations. Always check the temperature of the heat block before starting on a protocol in case you need to make any adjustments. If you need to change the temperature of a block, be sure the change it back …

helpful_sites_for_unm_classes

Anonymous (anonymous@undisclosed.example.com) — 2018-07-12T08:06:19+00:00

Organic Chemistry (CHEM301/302) Khan Academy videos can be super helpful. Leah 4 Sci has some great study sheets to help you drill reactions. Calculus This is a great site for math help. They have many examples and problem sets that are great for practice.

heparin_sodium_salt

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:10:52+00:00

Heparin sodium salt --- Ryla Cantergiani 2020/05/06 02:24 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. We have a 10 mg/mL and 50 mg/mL heparin solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

high-speed_centrifuge_tubes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:49:27+00:00

High-Speed Centrifuge Tubes --- Ryla Cantergiani 2020/04/20 22:48 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

high_copy_photo_stand

Anonymous (anonymous@undisclosed.example.com) — 2019-02-05T15:33:30+00:00

High Copy Photo Stand --- Christine Robbins 2019/02/05 15:26 Description ---------- The High Copy Photo Stand is used to take pictures of plates at the exact some height and location. This allows for consistent photos that are simple to edit and analyze. It is also used to mount webcams to film worms.

high_efficiency_yeast_transformation

Anonymous (anonymous@undisclosed.example.com) — 2018-07-20T17:00:55+00:00

High-Efficiency Yeast Transformation 2.0 Pre-Steps * Make sure that the 95C and 42C heat blocks are turned on and heated to the correct temperature before the beginning. * Remember to take the time to think about your controls before you begin (like a mock transformation and a mix transformation) For a mix transformation use an extra flip top tube and with a mock transformation follow the protocol for an extra strain of whatever the mock is and do not add DNA to it during Step 5.

hybridization_buffer

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:38:14+00:00

Hybridization Buffer --- Ryla Cantergiani 2020/06/11 15:37 Description ---------- DNA Hybridization Buffer facilitates spreading of probes and reduces drying of DNA during hybridization reactions. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

hybridization_wash

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:39:55+00:00

Hybridization Wash --- Ryla Cantergiani 2020/06/11 15:38 Description ---------- Hybridization wash aids in the removal of non-specific interactions between the probe and undesirable regions of the genome, thus allowing greater probe specificity. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

hydrochloric_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:13:07+00:00

Hydrochloric Acid --- Olivia Heath 2018/01/24 14:04 --- Ryla Cantergiani 2020/04/28 01:11 ---------- Description Hydrochloric Acid is a common strong acid. In lab, we will often use HCl for pH corrections when making media and reagents for experiments. When dealing with our HCl, you will most likely by using the pre-made 1 M HCl stock (on shelf ??).

hygromycin

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:11:23+00:00

Hygromycin --- Ryla Cantergiani 2020/06/08 21:05 Description ---------- Hygromycin is an antiobiotic. We use hygromycin resistance as a genetic marker. We keep a 50 mg/mL solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

hypoxia_chamber_setup

Anonymous (anonymous@undisclosed.example.com) — 2019-03-29T15:03:44+00:00

Budget Friendly Hypoxia/Anoxia Chamber -Olivia Heath 03/29/19 Overview ---------- This is a lab made hypoxic chamber to study the effects of hypoxia or anoxia in c. elegans. This set up was originally derived from this Jove article. Materials ---------- * Glass tupperware container with airtight lid. I recommend a lasagna pan to max out the n of the experiment.

ice_machine

Anonymous (anonymous@undisclosed.example.com) — 2019-02-07T13:59:23+00:00

Ice Machine --- Christine Robbins 2019/02/07 13:44 Description ---------- The ice machine is what, you guessed it, makes ice. The ice buckets live directly above it and should be used for any drug, cell or enzyme that needs to be maintained at low temperatures.

instructions

Anonymous (anonymous@undisclosed.example.com) — 2018-11-08T16:23:51+00:00

Instructions ---------- (use this template) Wiki Instructions Name of plugin Description link (end of template) ---------- Color Plugin that allows for simple colors in your wiki such as RED Documentation:

iptg

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:39:20+00:00

IPTG --- Olivia Heath 2018/02/07 15:27 ---------- Description Isopropyl β-D-1-thiogalactopyranoside We keep a 1M solution in the -20°C freezer. Formula: C9H18O5S [Link to MSDS] Location: , 4°C Fridge, stock in -20°C Freezer UNM Barcode: 117092 Notes and Warning:

isopropyl_alcohol

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:53:22+00:00

Isopropyl Alcohol --- Olivia Heath 2018/01/24 14:36 ---------- Description Isopropyl Alcohol Formula: C3H8O [Link to MSDS] Location: Cabinet 8 - under Hood UNM Barcode: 117076 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: BDH1133-1LP

journal_club_methods

Anonymous (anonymous@undisclosed.example.com) — 2018-07-12T17:33:52+00:00

Mass Spectrometry General description:

kanamycin_sulfate

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T17:45:37+00:00

Kanamycin Sulfate --- Olivia Heath 2018/02/07 15:42 --- Ryla Cantergiani 2020/05/07 17:44 ---------- Description Kanamycin Sulfate. We use Kanamycin resistance as a genetic marker. Formula: ‎C18H38N4O15S [Link to MSDS] Location: 4°C Fridge UNM Barcode: 117093 Notes and Warning:

kelli_kagay

Anonymous (anonymous@undisclosed.example.com) — 2019-10-31T22:55:42+00:00

Kelli Kagay PODZIEL ODPŁACAĆ Nuże dwulatek ponoć chować “niewybitne” musy. Natychmiast dwulatek możliwe czerpać “niziutkie” dyżury. • Aksjomat autorskie: alenkasm / 123RF Porwanie Masowe Lenore Skenazy, przeżyłam okrzyknięta najnieszczęśliwszą mamą Ameryki, po bieżącym gdy przystała bezpośredniemu 9 upalnemu dawniej potomkowi, na powrót do dachu kwintalem. Sierocemu, w Świeżym Jorku. Wkurzona, ufundowała tumult Free - Range Kids, więc w obszernym odtwarzaniu - niemowlęta wolnowybiegowe. Rok pon…

kimwipes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:32:45+00:00

Kimwipes --- Ryla Cantergiani 2020/04/23 23:29 Description ---------- Kimwipes are like lab tissues. They are thin, paper wipes that are useful for delicate tasks. You may use them for wiping down the foil covering 96-well plates with 95% ethanol, or for cleaning microscope slides before use. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file na…

l-alanine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:09:54+00:00

L-Alanine * -- Olivia Heath 2018/01/24 14:39 ---------- Description L-Alanine Formula: C3H7NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117037 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number: B00F8IGL20

l-arginine_base

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:11:39+00:00

L-Arginine Base --- Olivia Heath 2018/01/24 14:43 ---------- Description L-Arginine Base Formula: ‎C6H14N4O2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117038 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-asparagine

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:11:58+00:00

L-Asparagine * -- Olivia Heath 2018/01/24 14:47 ---------- Description L-Asparagine Formula: ‎C4H8N2O3 [Link to MSDS] Location: KK- Chemical Shelf UNM Barcode: 117045 Notes and Warning: Order Information ---------- Vendor: Fisher / Acros Catalog Number: AC401481000

l-aspartic_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:15:25+00:00

L-Aspartic Acid --- Olivia Heath 2018/01/24 16:51 ---------- Description L-Aspartic Acid Formula: C4H7NO4 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117036 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-cysteine_hcl_monobasic

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:19:44+00:00

L-Cysteine HCL Monobasic --- Olivia Heath 2018/01/24 17:24 ---------- Description L-Cysteine HCL Monobasic Formula: C3H7NO2S [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117032 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements

l-glutamic_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:32:06+00:00

L-Glutamic Acid --- Olivia Heath 2018/01/24 17:29 ---------- Description L-Glutamic Acid Formula: C5H9NO4 |Link to MSDS}} Location: WW- Chemical Shelf UNM Barcode: 117028 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements

l-glutamine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:31:24+00:00

L-Glutamine --- Olivia Heath 2018/02/07 13:28 ---------- Description L-Glutamine Formula: ‎C5H10N2O3 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117031 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-histidine_hcl

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:41:16+00:00

L-Histidine HCL --- Olivia Heath 2018/02/07 13:31 ---------- Description L-Histidine HCL Formula: C6H10ClN3O2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117039 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-isoleucine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:43:02+00:00

L-Isoleucine --- Olivia Heath 2018/02/07 13:33 ---------- Description L-Isoleucine Formula: C6H13NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117042 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-leucine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:41:33+00:00

L-Leucine --- Olivia Heath 2018/02/07 13:35 ---------- Description L-Leucine Formula: C6H13NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117040 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number: B00GW5ZHI2

l-lysine_hcl

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:41:00+00:00

L-Lysine HCL --- Olivia Heath 2018/02/07 13:37 ---------- Description L-Lysine HCL Formula: ‎C6H15ClN2O2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117030 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-methionine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:36:05+00:00

L-Methionine --- Olivia Heath 2018/02/07 13:40 ---------- Description L-Methionine Formula: ‎C5H11NO2S [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117041 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-phenylalanine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:35:29+00:00

L-Phenylalanine --- Olivia Heath 2018/02/07 13:42 ---------- Description L-Phenylalanine Formula: ‎C9H11NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117025 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-proline

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:35:45+00:00

L-Proline --- Olivia Heath 2018/02/07 13:45 ---------- Description L-Proline Formula: ‎C5H9NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117034 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number: B00F8I3I30

l-serine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:43:41+00:00

L-Serine --- Olivia Heath 2018/02/07 13:47 ---------- Description L-Serine Formula: C3H7NO3 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117035 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:B00JU75LN0

l-threonine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:45:55+00:00

L-Threonine --- Olivia Heath 2018/02/07 13:49 ---------- Description L-Threonine Formula: ‎C4H9NO3 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117033 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-tryptophan

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:44:12+00:00

L-Tryptophan --- Olivia Heath 2018/02/07 13:51 ---------- Description L-Tryptophan Formula: ‎C11H12N2O2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117027 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-tyrosine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:43:56+00:00

L-Tyrosine --- Olivia Heath 2018/02/07 13:54 ---------- Description L-Tyrosine Formula: ‎C9H11NO3 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117026 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

l-valine

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:43:18+00:00

L-Valine --- Olivia Heath 2018/02/07 13:56 ---------- Description L-Valine Formula: ‎C5H11NO2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117029 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number: B00H4HA930

lab_camera

Anonymous (anonymous@undisclosed.example.com) — 2020-04-08T18:13:48+00:00

Lab Camera --- Ryla Cantergiani 2020/04/08 17:49 Description ---------- The lab camera is a tool to take pictures of lab-related subjects. We use this camera to take pictures of tetrad plates, experimental set-ups, etc. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

lab_coats

Anonymous (anonymous@undisclosed.example.com) — 2020-03-25T21:48:01+00:00

Lab Coats --- Ryla Cantergiani 2020/03/25 21:22 Description ---------- Lab coats are worn to protect street clothes and exposed skin when doing experiments. Lab coats should be worn when dealing with hazardous chemicals. Enter description of the equipment, please link any protocols or reagent pages that use it.

lab_meeting_protocol

Anonymous (anonymous@undisclosed.example.com) — 2018-11-25T11:52:08+00:00

Lab Meeting Protocol --- Jacob Anderson 2018/11/25 11:29 Overview ---------- All lab meeting slides MUST go into dropbox as soon as you present. Also ALWAYS use the lab meeting slides template. The link is provided below in the “ALL THINGS INTERN”. If you are a member of this lab this should be easy to get to.

lab_printer

Anonymous (anonymous@undisclosed.example.com) — 2020-04-02T23:12:57+00:00

Lab Printer --- Ryla Cantergiani 2020/04/02 22:56 Description ---------- We currently use a Brother HL-3170CDW Digital Color Printer. Use this to print out protocols, papers, etc. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

lab_rules_to_live_by

Anonymous (anonymous@undisclosed.example.com) — 2020-04-02T23:31:16+00:00

Lab Rules to Live By These are standard lab rules that everyone should know. If something is listed here, then it is a specific, very serious pet peeve of at least one person who works in this lab currently, and whatever amount of extra work it appears to entail is FAR FAR LESS than the hassle you will have for not sticking to these rules.

lab_tape

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:24:10+00:00

Lab Tape --- Ryla Cantergiani 2020/04/20 21:18 Description ---------- Lab tape is labeling tape. It is more for labeling than fastening things. We have lab tape in several colors: red, orange, yellow, green, blue, pink, white. This can be especially useful if you are color-coding. For best results, use a thick sharpie when labeling.

labeling_a_gel

Anonymous (anonymous@undisclosed.example.com) — 2025-09-05T13:48:32+00:00

Labeling a Gel * Make a new folder with the date, who done each thing, and what the gel is. * Put the original image in the folder. * Open with GIMP * Image > Transform > Rotate 90 ° clockwise * Tools > Selection Tools > Rectangle select

large_plates_10cm

Anonymous (anonymous@undisclosed.example.com) — 2020-04-24T00:14:29+00:00

Large Plates (10 cm) --- Ryla Cantergiani 2020/04/24 00:11 Description ---------- We use large plates for our plate media, such as NGM, YPD, C-Ura, etc. When inside their bag, they are still sterile. Do NOT place your hands/arms inside the bags - we need them to remain sterile so we can use them to bag media plates.

laser_thermometer

Anonymous (anonymous@undisclosed.example.com) — 2020-04-03T00:01:31+00:00

Laser Thermometer --- Ryla Cantergiani 2020/04/02 23:52 Description ---------- Laser thermometers are used to measure temperature without direct contact. This is especially useful when measuring the temperature of sterile products, such as media. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

lb_and_variations

Anonymous (anonymous@undisclosed.example.com) — 2020-04-10T20:55:54+00:00

LB and Variations --- Ryla Cantergiani 2020/04/10 19:46 Description ---------- LB (originally, Lysogeny Broth, now often called Luria Broth or Luria Bertani) is a liquid medium for growing bacteria. You may use LB when pulling bacterial strains from the freezer, overnighting bacterial strains, or making a new stock of OP-50. When overnighting bacterial, take what you need using a pipet aide and stripette. When making a new OP-50 stock, use an fresh 250 mL bottle of LB.

levamisole

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:10:35+00:00

Levamisole --- Olivia Heath 2018/02/07 14:40 ---------- Description Levamisole We keep a 1 mM solution in the -20°C freezer. Formula: ‎C11H12N2S [Link to MSDS] Location: LL- Chemical Shelf UNM Barcode: 117070 Notes and Warning: Order Information ----------

lithium_acetate_dihydrate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:25:57+00:00

Lithium Acetate dihydrate --- Christine Robbins 2018/01/23 13:13 Description ---------- We keep a 1M LiOAc solution on Shelf O. Formula: LiO2C2H3 ·2H2O [Link to MSDS] Location: Shelf LL UNM Barcode: 117004 Notes and Warning: Order Information ---------- Vendor: VWR

m9_media

Anonymous (anonymous@undisclosed.example.com) — 2020-04-06T22:57:38+00:00

M9 Description ---------- M9 solution is used when working with worms. It is used in Freezing Worm Strains. [Insert image of media here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

magnesium_chloride_hexahydrate

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:18:54+00:00

Magnesium chloride hexahydrate --- Christine Robbins 2018/01/23 13:21 Description ---------- Formula: MgCl2 ·6H2O [Link to MSDS] Location:Shelf LL UNM Barcode: 117005 Notes and Warning: Order Information ---------- Vendor: himediastore.com Part Number: GRM3922-500G

magnesium_sulfate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:27:46+00:00

Magnesium Sulfate --- Christine Robbins 2018/01/23 13:28 Description ---------- Magnesium Sulfate is used to make 1M MgSO4, a component of NGM plates. We keep a pre-made 1M stock on Shelf R. Formula: MgSO4 [Link to MSDS] Location:Shelf LL UNM Barcode: 117006 Notes and Warning:

magnetic_hot_plates

Anonymous (anonymous@undisclosed.example.com) — 2020-04-06T23:33:00+00:00

Magnetic Hot Plate --- Ryla Cantergiani 2020/04/06 23:05 Description ---------- Magnetic hot plates can simultaneously stir and heat liquids. They are used when making media or other solutions. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

making_liquid_media

Anonymous (anonymous@undisclosed.example.com) — 2018-11-14T15:37:13+00:00

Making Liquid Media --- Christine Robbins 2018/11/14 14:00 Description ---------- Below are general directions for making liquid media. Please review them whenever making liquid stocks of media. Notes: * Whenever using media, please remember that if you open the last bottle, you need to make more right away. Not later. If you are a student and you must leave for class, please let your friendly neighborhood Lab Tech or Lab Aide know that the media needs to be made

making_op-50_stock

Anonymous (anonymous@undisclosed.example.com) — 2020-04-10T22:12:43+00:00

Making an OP-50 Stock --- Ryla Cantergiani 2020/04/10 21:42 Overview ---------- We use OP-50 stocks for seeding NGM plates. Unfortunately, while LB media is great for growing up OP-50, it's also great for growing up all sorta of other contaminants as well. If you see gross floaties in the stock, or suspect your stock is contaminated, you'll need to make a new stock.

maleic_acid_buffer

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:15:11+00:00

Maleic Acid Buffer --- Ryla Cantergiani 2020/06/11 15:14 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

mef_crispr_ko

Anonymous (anonymous@undisclosed.example.com) — 2021-03-17T18:49:13+00:00

Overview The MEFs that we have in the lab (as of 2021) are Wildtype (Purchased, ATCC), GCN2 KO (Purchased, ATCC), and ATF4 KO (using IDT CRISPR, in-house). This protocol aims to generate a step-by-step explanation on how to create a knockout MEF cell line with applicability to other cell culture lines.

mef_freeze_thaw_procedure

Anonymous (anonymous@undisclosed.example.com) — 2021-07-26T17:10:09+00:00

Overview MEFs are frozen down in a nitrogen freezer (located in the Hines lab). There, they can be stored for very long periods of time while maintaining viability. Cells can be stored in the -80 freezer for up to one week. All reagents used to create MEF growth media should not be open outside a BSL-2 certified hood. Reserve a time for these hoods and follow all Aseptic Techniques outlined here on the wiki in previous protocols. All contamination in cell culture happens because of improper t…

mef_image_data_labeling_in_r

Anonymous (anonymous@undisclosed.example.com) — 2021-09-08T17:42:56+00:00

Overview After performing an experiment involving the Mef subculturing 24-well plate protocol or similar, the wells must be imaged and the proliferation data harvested and processed in RStudio. This protocol will outline how to use an EVOS FL Auto to image cell culture well plates that have been stained with a Hoescht blue stain, as well as how to harvest and process relevant cell proliferation data from those images in RStudio.

mef_quickextract_dna_extraction_procedure

Anonymous (anonymous@undisclosed.example.com) — 2021-03-22T14:41:32+00:00

Overview To perform DNA extraction on MEF cells, use QuickExtract. QuickExtract will detach and destroy the adherent MEFs cultured on a tissue culture plate, and after this, ~10-minute protocol, the DNA in the mixture will be ready for PCR. Protocol

mef_subculturing

Anonymous (anonymous@undisclosed.example.com) — 2021-08-04T15:09:40+00:00

Overview MEF cells are an adherent mammalian cell line. The ones that are currently being used are immortalized. These cells grow rapidly and need consistent “splitting,” or “subculturing.” Typically, adherent mammalian cell lines are to be subcultured when they reach 70-80% confluence. More than this could result in poor cell viability in subsequent subcultures, or genetic changes and deviation of the cell type to another phenotype. To ensure the most accurate results from your experiments, yo…

mef_subculturing_24-well_plate

Anonymous (anonymous@undisclosed.example.com) — 2021-07-08T16:21:07+00:00

mef_viafect_plasmid_transfection

Anonymous (anonymous@undisclosed.example.com) — 2021-07-21T22:04:25+00:00

Overview The best transfection reagent that Blaise tried in his plasmid-transfection escapade to collect Dual-Luciferase and fluorescence-reporter data for the AFAR 2021 grant in MEF cells was ViaFect by Promega: . The other methods attempted included: NEON electroporation, FuGene HD. If ViaFect was not able to be used for some reason, then the next thing that I would…

metformin_hydrochloride

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:19:43+00:00

Metformin Hydrochloride --- Olivia Heath 2018/02/07 14:19 ---------- Description Metformin Hydrochloride Formula: ‎C4H12ClN5 [Link to MSDS] Location: 59- Dark Chemicals UNM Barcode: 117046 Notes and Warning: Order Information ---------- Vendor: NO DATA Catalog Number:

methylene_blue

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:01:58+00:00

Methylene Blue --- Ryla Cantergiani 2020/06/11 16:00 Description ---------- Methylene blue is a common stain. We keep a 0.2% solution on Shelf Q. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

microscope_slides_and_covers

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:31:35+00:00

Microscope Slides and Covers --- Ryla Cantergiani 2020/04/20 22:23 Description ---------- These are new microscope slides and covers to make your own specimen slides. Although they are supposed to be pre-cleaned, it's a good idea to wipe them down with 70% ethanol and a kimwipe to remove any streaks or particulates.

microwave

Anonymous (anonymous@undisclosed.example.com) — 2019-02-07T14:09:20+00:00

Microwave --- Christine Robbins 2019/02/07 14:01 Description ---------- The lab microwave is used in order to melt agarose for makeing agarose gels for electrophoresis. Location:Below shelf YY Notes and Warning: DO NOT microwave any containers with caps or lids. DO NOT place any food items in the microwave. Please use the heat resistant gloves whenever removing hot liquids from the microwave.

midi_prep

Anonymous (anonymous@undisclosed.example.com) — 2018-11-21T16:04:51+00:00

Midi Prep --- Christine Robbins 2018/05/01 13:00 --- edited: --- Dan Felker 2018/11/05 13:21 Description ---------- We use Qiagen's Midi Prep kit in order to extract plasmids out of bacterial cells. Please remember to plan ahead and make sure we have everything you will need to complete the protocol. This protocol also takes between 3 and 5 hours and there is

milliq_water

Anonymous (anonymous@undisclosed.example.com) — 2018-03-09T12:15:15+00:00

MilliQ Water

mini_prep

Anonymous (anonymous@undisclosed.example.com) — 2018-11-20T12:59:56+00:00

Qiagen Mini Prep [Insert Signature here. You must be signed in to do this] Overview ---------- This protocol is designed for purification of up to 20 µg of high-copy plasmid DNA from 1–5 ml overnight cultures of E. coli in LB medium. Materials

mini_worm_plates

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T22:30:51+00:00

Mini Worm Plates --- Ryla Cantergiani 2020/04/23 22:23 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

mounting_worms_for_imaging

Anonymous (anonymous@undisclosed.example.com) — 2024-02-07T18:03:19+00:00

Materials: * colored lab tape * glass slides * cover glass * fast-dry clear nail polish * microscope slide book * 2% agarose * 70% EtOH * Kim Wipes Steps: * Clean and dry slides and cover glass to be used. Note: only 70% EtOH and Kim Wipes should be used to clean these.

mouse_embryonic_fibroblast_growth_media

Anonymous (anonymous@undisclosed.example.com) — 2021-02-12T16:16:56+00:00

Mouse Embryonic Fibroblast (MEF) Growth Media Recipe Overview MEFs can be grown in vitro on Tissue Culture (TC) treated flasks with growth media based on Dulbecco's Modified Eagle Medium and Fetal Bovine Serum. Growth media is supplemented with other factors, and freeze-down media is supplemented with DMSO. TC treated flasks have more hydrophilic properties than non-TC treated flasks and are important when growing adherence cell lines. MEFs are adherence cells.

mupirocin

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:45:00+00:00

Mupirocin --- Olivia Heath 2018/02/07 14:13 ---------- Description Mupirocin is an antibiotic. We keep a 50 mg/mL and 540 mg/mL stock in the -20°C freezer. Formula: C26H44O9 [Link to MSDS] Location: H- Chemical Shelf UNM Barcode: 117008 Notes and Warning: Store at RT

myo-inositol

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:36:29+00:00

Myo-Inositol --- Olivia Heath 2018/02/07 14:16 ---------- Description Myo-Inositol Formula: ‎C6H12O6 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117044 Notes and Warning: Order Information ---------- Vendor: Ebay / lifeline nutrients Catalog Number:

ngm_plates_and_variations

Anonymous (anonymous@undisclosed.example.com) — 2022-11-11T16:23:08+00:00

NGM (Nematode Growth Media) Plates Media Protocol (Makes 2 Liters) 1) Clean benchtop station by magnetic stirrers with 70% ethanol 2) Retrieve materials (ALL MUST BE STERILE IE. MARKED WITH BLACKENED AUTOCLAVE TAPE): [1] 4 Liter Flask (Top Glassware Shelf) OR [2] 2 Liter Flasks [3] Weigh Boats (Drawer 61) [3] Spatulas (Drawer 59) [1] Stir Bar (Drawer 59) [1] 500 milliliter graduated cylinder OR 1 Liter graduated cylinder (Top Glassware Shelf) Electronic Sca…

niaid

Anonymous (anonymous@undisclosed.example.com) — 2018-08-09T18:07:40+00:00

non-unm_programs

Anonymous (anonymous@undisclosed.example.com) — 2022-11-04T16:48:33+00:00

Columbia University BEST Program - Summer program at Columbia University. Students will spend the summer learning about research in biostatistics and cardiovascular and pulmonary diseases Gateways to the Laboratory Summer Program - MD-PhD training summer program for students finishing their freshman or so…

nourseothricin

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:10:23+00:00

Nourseothricin (NAT) --- Ryla Cantergiani 2020/05/07 17:36 Description ---------- Nourseothricin (also known as NAT) is an antibiotic. We use NAT resistance as a genetic marker. We keep a 100 mg/mL solution in the -20°C freezer. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

nsf_grfp

Anonymous (anonymous@undisclosed.example.com) — 2018-10-12T17:44:33+00:00

General Website: Additional external resources: Reality of personal statement struggles:

nutrient_broth

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:20:37+00:00

Nutrient Broth --- Christine Robbins 2018/01/23 13:33 Description ---------- Nutrient Broth is used to make GNA plates for yeast sporulation. Formula: N/A [Link to MSDS] Location:Shelf LL UNM Barcode: 117007 Notes and Warning: Order Information ---------- Vendor: Bio-world.com / bioPLUS

overnight_bacterial_strains

Anonymous (anonymous@undisclosed.example.com) — 2018-11-16T13:34:40+00:00

Overnighting Bacteria --- Jacob Anderson 2018/11/16 13:32 Overview ---------- The purpose of this experiment is to propagate a bacterial strain for further experimentation. You may need to do a transformation or run a midi or mini - prep for measure the concentration of DNA. Either way follow these basic steps.

overnight_yeast_strains

Anonymous (anonymous@undisclosed.example.com) — 2018-11-01T13:04:15+00:00

Overnight Yeast Strains Overview ---------- When we need to use a specific strain of yeast for a protocol, we first must grow it up overnight. Whether the strain is frozen in the -80°C Freezer or fresh on a yeast media plate, the process of overnighting is the same. Materials

para-aminobenzoic_acid_paba

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:42:29+00:00

PABA --- Olivia Heath 2018/02/20 13:13 ---------- Description Para-AminoBenzoic Acid Formula: H2NC6H4CO2H [Link to MSDS] Location: -20°C Freezer - also WW Chemical Shelf UNM Barcode: 117043 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements

parafilm

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:27:25+00:00

Parafilm --- Ryla Cantergiani 2020/04/23 23:23 Description ---------- Parafilm is a flexible material made out of waxes and polyolefins. While it has many uses, its most common use in our lab is sealing plates to prevent them from drying out of getting contaminated. It stretches when pulled. One full width strip can seal 2-3 large plates.

pasteur_pipettes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:47:24+00:00

Pasteur Pipettes --- Ryla Cantergiani 2020/04/20 22:46 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

pcr_strips_8-well

Anonymous (anonymous@undisclosed.example.com) — 2020-04-24T00:00:02+00:00

PCR Strips (8-Well) --- Ryla Cantergiani 2020/04/23 23:55 Description ---------- PCR strips are made for use in the PCR machine (thermocycler). They come in strips of 8, with separate cap strips. They are used for running boils and PCRs during the PCR protocol. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

peg_transformation_solution

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:25:08+00:00

PEG Transformation Solution --- Ryla Cantergiani 2020/06/08 21:48 Description ---------- Polyethylene glycol (PEG) transformation solution is used to mediate transformations. We keep a 50% PEG solution on Shelf O. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

peptone

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:21:02+00:00

Peptone --- Olivia Heath 2018/02/07 11:58 ---------- Description Apex Peptone Formula: ‎N/A [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117009, 117054, 117055,117056 Notes and Warning: Order Information ---------- Vendor: Genesee/Apex Catalog Number:

ph_meter

Anonymous (anonymous@undisclosed.example.com) — 2020-04-03T00:11:47+00:00

pH Meter --- Ryla Cantergiani 2020/04/03 00:06 Description ---------- pH Meter. Instructions on using the pH meter here. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

pipette_aide

Anonymous (anonymous@undisclosed.example.com) — 2020-04-03T00:38:03+00:00

Pipette Aide --- Ryla Cantergiani 2020/04/03 00:12 Description ---------- Pipette aides are used when transferring larger volumes of liquids, such as pouring plates. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

pipette_tips

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T22:23:21+00:00

Pipette Tips --- Ryla Cantergiani 2020/04/13 14:51 Description ---------- Pipette tips are disposable attachments for our pipettes. There are several different sizes that correspond to the different sized pipettes. * P2 pipettes use P10 tips. * P10 pipettes use P10 tips.

pipettes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-13T15:07:05+00:00

Pipettes --- Ryla Cantergiani 2020/04/06 21:47 Description ---------- Pipettes are used to transfer small volumes of liquid. We have several sizes of pipette: P2, P10, P20, P200, and P1000. Before using pipettes for experiments, make sure you complete the [ Pipette Practice Worksheet]! Just a few reminders when pipetting:

platinum_wire

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:43:07+00:00

Platinum Wire --- Ryla Cantergiani 2020/04/20 22:41 Description ---------- Worms picks use platinum wire. Platinum can be re-heated over and over to sterilize without losing its shape. Platinum wire is relatively fragile, so care must be used when shaping. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

pmsf

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T16:30:53+00:00

PMSF --- Olivia Heath 2018/02/07 16:29 ---------- Description PMSF Formula: C7H7FO2S |Link to MSDS}} Location: -20°C Freezer UNM Barcode: 117106, 117107 Notes and Warning: Order Information ---------- Vendor: Fisher Scientific Catalog Number: 50-121-7869

pnpp

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:34:52+00:00

PNPP --- Ryla Cantergiani 2020/04/28 02:34 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

polyethylene_glycol

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:21:31+00:00

Polyethylene Glycol * -- Olivia Heath 2018/02/07 12:07 ---------- Description L-Arginine Base Formula: ‎C6H14N4O2 [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117012 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: TCP0885-aa500G

polysome_lysis_buffer

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:57:33+00:00

Polysome Lysis Buffer --- Ryla Cantergiani 2020/06/08 21:56 Description ---------- Polysome lysis buffer for yeast. We keep 1x and 10x buffers in lab. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

potassium_acetate_anhydrous

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:21:48+00:00

Potassium Acetate Anhydrous --- Olivia Heath 2018/02/07 14:10 ---------- Description Potassium Acetate Anhydrous Formula: C2H3KO2 [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117082 Notes and Warning: Order Information ---------- Vendor: himediastore.com

potassium_chloride

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:22:15+00:00

Potassium Chloride * -- Olivia Heath 2018/02/07 12:19 ---------- Description L-Arginine Base Formula: ‎KCl [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117014 Notes and Warning: Order Information ---------- Vendor: VWR/Aldon Catalog Number: 470302-092 / PP0460-500GR

potassium_citrate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:37:41+00:00

Potassium Citrate --- Ryla Cantergiani 2020/06/09 17:36 Description ---------- We keep a pre-made potassium citrate buffer (pH=6) on Shelf R. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

potassium_hydroxide

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:20:52+00:00

Potassium Hydroxide * -- Olivia Heath 2018/02/07 12:22 ---------- Description Potassium Hydroxide pellets may be used when making reagents. We also have a 5M KOH stock (on shelf O). Formula: ‎KOH [Link to MSDS] Location: MM - Chemical Shelf UNM Barcode: 117013 Notes and Warning:

potassium_phosphate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:29:24+00:00

Potassium Phosphate * -- Olivia Heath 2018/02/07 12:27 ---------- Description Potassium Phosphate Monobasic [KH2PO4] 99+% Fine Crystals 1.5 Lb in Three Space-Saver Bottles. We keep a pre-made potassium buffer solution (pH=6) on Shelf R. Formula: ‎KH2PO4

practicing_with_worms_on_the_scope

Anonymous (anonymous@undisclosed.example.com) — 2020-04-13T22:18:52+00:00

Practicing with Worms on the Scope --- Ryla Cantergiani 2020/04/13 16:13 Overview ---------- Before you can score any worm lifespans, you need to be comfortable working with worms. Adjusting the worm scope, picking up and putting down worms, and not poking holes will take some practice.

protein_extraction

Anonymous (anonymous@undisclosed.example.com) — 2021-08-15T20:48:50+00:00

Protein Extraction (Yeast) --- Ryla Cantergiani 2021/08/15 18:13 Overview ---------- This protocol is for the preparation of protein extracts in yeast. Protein extracts may be used for proteomics analysis, such as with mass spectroscopy. Protocol adapted from the Clontech Yeast Protocols Handbook (2009).

proteinase_k

Anonymous (anonymous@undisclosed.example.com) — 2020-06-08T21:35:27+00:00

Proteinase K --- Olivia Heath 2018/02/07 15:46 ---------- Description Proteinase K We keep a 20 mg/mL solution in the -20°C freezer. Formula: ‎N/A [Link to MSDS] Location: -20°C Freezer UNM Barcode: 117094 Notes and Warning: Order Information ----------

protocols

Anonymous (anonymous@undisclosed.example.com) — 2026-05-14T17:07:05+00:00

Protocols ---------- Media and Stock Solutions ---------- Liquid Media Recipes Making Liquid Media Yeast Media YEP Media and Variations SC, SD, SD(-N), and Dropout Media Min, Min D+, Min G+ Bacteria Media LB and Variations Worm Media M9 Media Worm S-Basic Work S-Complete Worm Freezing Solution Solid Media Recipes Making Solid Media and Pouring Plates Yeast Plates YEP Plates and Variations SC, and Dropout Plates Basic Plates Basic +HLU Plates GNA Plates Bacteria Plates L…

pulling_bacterial_strains_from_freezer

Anonymous (anonymous@undisclosed.example.com) — 2020-04-13T15:43:14+00:00

Pulling Bacterial Strains --- Ryla Cantergiani 2020/04/13 14:38 Overview ---------- We have our bacterial strains frozen in the -80C freezer. When you need to use a strain, you'll have to pull it and grow it up first. The bacteria will take time to grow up, so plan ahead when organizing your experiments.

pulling_yeast_from_the_freezer

Anonymous (anonymous@undisclosed.example.com) — 2018-11-05T18:19:55+00:00

Name of Protocol Overview ---------- Materials ---------- Procedure ---------- 1) When pulling strains from -80°C Freezer, make sure you wear both Nitrile gloves and polar bear gloves. 2) Determine which boxes contain the needed strains by referencing the freezer map on the outer door of -80°C Freezer. Notes

putting_in_a_service_request

Anonymous (anonymous@undisclosed.example.com) — 2018-02-08T18:18:16+00:00

Putting in a Service Request Go to this page: Select “Submit a Service Request” from the panel on the right. Select your campus (We are Albuquerque). Select your building (We are 253 Biomedical Research Facility). Fill in all other information on the next page (We will use area 2 maintenance for any kind of electrical and plumbing needs and select custodial for any kind of paper towel and trash needs) and hit submit. You may leave the index number b…

putting_in_a_work_order

Anonymous (anonymous@undisclosed.example.com) — 2018-02-08T18:17:13+00:00

qiagen_plasmid_midi_kit

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T13:19:04+00:00

Quiagen Plasmid Midi Kit --- Olivia Heath 2018/02/20 13:17 ---------- Description Quiagen Plasmid Midi Kit Formula: N/A [Link to MSDS] Location: -20°C Freezer UNM Barcode: 117050 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements

rapamycin

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T17:01:11+00:00

Rapamycin --- Olivia Heath 2018/02/07 16:56 ---------- Description Rapamycin Formula: ‎C51H79NO13 [Link to MSDS] Location: G- Chemical Shelf UNM Barcode: 117101 Notes and Warning: Order Information ---------- Vendor: Fisher Scientific Catalog Number: NC9248324

reagent_troughs

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:14:13+00:00

Reagent Troughs --- Ryla Cantergiani 2020/04/20 22:11 Description ---------- Reagent troughs are used to hold a small amount of reagent for easier access, such as when using a multichannel pipette. They hold 25 mL. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

reagents

Anonymous (anonymous@undisclosed.example.com) — 2019-11-05T10:10:26+00:00

Reagents and Consumables Reagents and consumables are items in the lab that are used up and need to be re-made or re-ordered. This includes all chemicals, medias, and single use lab plastics/tips/etc. Instructions When adding a new item to the reagents sheet, always create a new page below under the

reagents_and_consumables

Anonymous (anonymous@undisclosed.example.com) — 2021-01-11T16:38:38+00:00

Reagents and Consumables Reagents and consumables are items in the lab that are used up and need to be re-made or re-ordered. This includes all chemicals, media, and single-use lab plastics/tips/etc. Instructions: When adding a new item to the reagents and consumables sheet, always create a new page below under the correct section, placing the item in the correct alphabetical order. Always use the appropriate template page when creating a page. Templates can be found

refilling_ethanol_bottles

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T01:51:48+00:00

Refilling Ethanol Bottles --- Ryla Cantergiani 2020/04/28 01:21 Overview ---------- We use 70% and 95% stocks of 200-proof ethanol to sterilize our bench spaces and several pieces of equipment in lab. Squeeze bottles of 70% and 95% are on every bench, and they are refilled as part of Friday Clean-Up tasks (or when needed).

repeater_pipette

Anonymous (anonymous@undisclosed.example.com) — 2022-11-11T16:19:12+00:00

Repeater Pipette --- Ryla Cantergiani 2020/04/06 22:12 Description ---------- Repeater pipettes are used for repeatedly dispensing the same volume of liquid. We use these when manually pipetting over and over is more work than it's worth, such as pouring small NGM plates or seeding small NGM plates.

repeater_pipette_tips

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T22:45:26+00:00

Repeater Pipette Tips --- Ryla Cantergiani 2020/04/23 22:43 Description ---------- We have six sizes of repeater tips: 0.1mL, 1mL, 5mL, 10mL, 25mL, 50mL. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

replica_plating

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T16:28:29+00:00

Replica Plating --- Ryla Cantergiani 2020/03/30 16:28 Overview ---------- Replica Plating is generally, a process of stamping a replica of a set of colonies, spots, or streaks from one agar petri dish (“plate”) to another, usually from a general growth media onto one or more marker or selective media.

replica_plating_and_scoring_tetrads

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T16:29:02+00:00

Tetrad Scoring [Insert Signature here. You must be signed in to do this] Overview ---------- Enter description of the media, please link any protocols that it is used in. In this section you put why we do this protocol and how it works very generally,as well as what the protocol(s) immediately preceding this and immediately following this are (with wiki links), if applicable.

replica_plating_tool

Anonymous (anonymous@undisclosed.example.com) — 2020-10-05T18:30:07+00:00

Description ---------- The Replica Plating Tool uses a sheet of velvet cloth on a cylindrical metal press with a circular band that allows for copying of certain yeast strains to more plates. You push down the sheet of velvet on the cylindrical press using the circular band to keep the velvet in place. You then push the inside of the plate of yeast gently on the velvet to allow for the velvet to pick up the yeast needed. Finally, you take your empty plates that you need to replicate and gently…

reserving_brf_218

Anonymous (anonymous@undisclosed.example.com) — 2018-07-25T17:41:23+00:00

To reserve room BRF 218 contact Sharon Pruitt(SPruitt@salud.unm.edu) or Evangelina Munoz (EValenzuela@salud.unm.edu) providing them the time(s) you would like the room for and then you can get the key from the main office Fitz 249

rnase_free_spray

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:51:59+00:00

RNAse FREE --- Olivia Heath 2018/02/20 13:01 ---------- Description RNase FREE, 475ml Spray Bottle Formula: N/A [Link to MSDS] Location: Shelf S UNM Barcode: 117095 Notes and Warning: Order Information ---------- Vendor: Genesee Catalog Number: 10-228 Order Link

rnase_free_water

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:51:24+00:00

RNAse FREE Water --- Ryla Cantergiani 2020/04/20 22:50 Description ---------- RNAse free water has no RNAse enzymes so that it will not break down RNA. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

rubber_bands

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:21:40+00:00

Rubber Bands --- Ryla Cantergiani 2020/04/20 22:19 Description ---------- We use rubber bands to hold stacks of plates together. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

s-cap_travel_award

Anonymous (anonymous@undisclosed.example.com) — 2018-11-30T18:51:00+00:00

Travel award for graduate or undergraduate students to cover expenses for one professional or academic conference. Follow link for more information and application materials.

salmon_sperm

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T13:11:53+00:00

Salmon Sperm --- Olivia Heath 2018/02/20 13:01 ---------- Description Salmon Sperm Formula: N/A Location: -20°C Freezer UNM Barcode: N/A Notes and Warning: Order Information ---------- Vendor: Fisher Catalog Number: 96105D Order Link

sc_and_dropout_plates

Anonymous (anonymous@undisclosed.example.com) — 2018-09-27T13:32:10+00:00

To make dropout plates you need to measure out: * 1.7g Yeast nitrogen Base (the one without ammonium sulfate or nutrients) * 5g Ammonium sulfate * 20g Sunrise (or BD) Agar * 1.4g of the premixed SC or dropout powder * all of which is mixed in 1L of water

sc_and_dropout_powders

Anonymous (anonymous@undisclosed.example.com) — 2018-06-26T16:57:16+00:00

Ingredient List:

sc_dropout_powder

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:55:49+00:00

SC Dropout Powder --- Ryla Cantergiani 2020/05/07 18:53 Description ---------- Synthetic complete (SC) dropout powder contains the amino acids necessary for yeast growth. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

sc_sd_sd_-n_and_dropout_media

Anonymous (anonymous@undisclosed.example.com) — 2022-10-17T14:41:56+00:00

C-URA (3L) solid media! If you're making liquid, omit the agar. 0.) This can be altered for C-whatever by substituting the appropriate amino acid powder in the last ingredient of step 3. p.s. SC-ura is the same thing as C-ura, etc. 1.) Add 1.5L of water to a 4L flask and place on a magnetic stirrer / hot plate.

scholarships_grants_and_fellowships

Anonymous (anonymous@undisclosed.example.com) — 2018-08-09T18:06:34+00:00

Grad Student Undergrad. Students Postdoc

scoops

Anonymous (anonymous@undisclosed.example.com) — 2020-04-06T22:50:25+00:00

Scoops --- Ryla Cantergiani 2020/04/06 22:38 Description ---------- We use scoops when dealing with solid reagents, such as when making media. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

scoring_tetrads

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T16:40:10+00:00

Tetrad Scoring --- Ryla Cantergiani 2020/03/30 16:30 Overview ---------- Scoring of yeast tetrads after sporulation is a crucial step in yeast strain construction. Because the four spores in a single tetrad are unordered products of a single meiosis, a lot can be gleaned right away from looking at the markers. Typically, you will have performed

screw_top_tubes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-24T00:14:00+00:00

Screw Top Tubes --- Ryla Cantergiani 2020/04/24 00:02 Description ---------- Screw top tubes are used for freezing strains in the -80°C freezer. They are 1.5 mL in volume. These tubes also have a niblet, which goes on top of the cap. These tubes should be labeled with a tough spot.

seeding_ngm_plates

Anonymous (anonymous@undisclosed.example.com) — 2022-12-19T14:50:45+00:00

Seeding Large NGM Plates * 2 600 mL Beakers * 200% EtOH * OP-50 * Hockey Stick * Put 50-100ml 200% EtOH and OP-50 in separate beakers. * Put on flame for sterilization and put hockey stick over flame until hot. * Dip hockey stick in 50-100ml EtOH and over flame again(wait for flame).

sequencing_tubes_eurofins

Anonymous (anonymous@undisclosed.example.com) — 2021-03-25T17:22:38+00:00

Sequencing Tubes (Eurofin) --- Ryla Cantergiani 2020/04/20 21:05 Overview This protocol will outline how to submit sequencing to Eurofins using the sequencing tubes provided. Protocol * Take your PCR product and clean and concentrate it with DNA concentration kit.

serological_pipettes

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:18:58+00:00

Serological Pipettes --- Ryla Cantergiani 2020/04/20 22:16 Description ---------- Serological pipettes attach to pipette aids to transfer large volumes of liquid. They are disposable. We have several sizes: 5mL, 10mL, 25mL, and 50mL. Enter description of the equipment,

single_worm_lysis_and_pcr

Anonymous (anonymous@undisclosed.example.com) — 2025-09-05T14:45:04+00:00

Single Worm Lysis and PCR --- Madison Otero 2025/08/14 13:07 Overview ---------- Purpose: to amplify regions of interest of DNA. Materials ---------- PK Buffer PCR Master Mix dH2O dH2O 20 mg/mL PK 10X Buffer 10X worm buffer

small_ngm_plates

Anonymous (anonymous@undisclosed.example.com) — 2023-01-20T16:50:22+00:00

Small NGM (Nematode Growth Media) Plates Media Protocol (Makes 1/2 Liter) 1) Clean benchtop station by magnetic stirrers with 70% ethanol 2) Retrieve materials (ALL MUST BE STERILE IE. MARKED WITH BLACKENED AUTOCLAVE TAPE): [1] 1 Liter Flask (Top Glassware Shelf) [3] Weigh Boats (Drawer 61) [3] Spatulas (Drawer 59) [1] Stir Bar (Drawer 59) [1] 500 milliliter graduated cylinder Electronic Scale (Drawer 61)

sodium_acetate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:12:36+00:00

Sodium Acetate --- Ryla Cantergiani 2020/06/11 16:04 Description ---------- Sodium acetate is the sodium salt of acetic acid. We keep pre-made 1M and 3M solution on Shelf Q. We also have a pre-made sodium acetate buffer on Shelf Q. We have a pre-made sodium acetate running buffer on Shelf Q. Sodium acetate running buffer is used for RNA electrophoresis for Northern blots.

sodium_azinde

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:24:09+00:00

Sodium Azinde --- Olivia Heath 2018/01/24 14:43 ---------- Description Sodium Azinde Formula: ‎NaN3 [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117038 Notes and Warning: Order Information ---------- Vendor: Amazon/ Bulk Supplements Catalog Number:

sodium_chloride

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:24:33+00:00

Sodium Chloride --- Olivia Heath 2018/02/07 12:36 ---------- Description Sodium Chloride Formula: NaCl [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117015 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: BDH9286-2.5KG Order Link:

sodium_hydroxide

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T16:21:10+00:00

Sodium Hydroxide --- Olivia Heath 2018/02/07 12:38 --- Ryla Cantergiani 2020/06/09 16:17 ---------- Description Sodium Hydroxide pellets. Used when making reagents/media. We have pre-made NaOH solutions you may use, such as for pH adjusting. We have 1M, 50%, and 10M NaOH solutions on Shelf O.

sodium_hypochlorite_6

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T14:13:19+00:00

Sodium Hypochlorite 6% --- Olivia Heath 2018/02/20 14:10 ---------- Description Calix - 6% Sodium Hypochlorite Solution x 500ml Formula: NaClO Location: 4°C Fridge UNM Barcode: 117108 Notes and Warning: Order Information ---------- Vendor: ebay

sodium_phosphate

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T16:10:37+00:00

Sodium Phosphate --- Ryla Cantergiani 2020/06/11 16:09 Description ---------- We have a pre-made sodium phosphate buffer (pH=7.4) on Shelf Q. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

software

Anonymous (anonymous@undisclosed.example.com) — 2022-11-04T17:00:39+00:00

Software These are some of our workhorse day to day programs in the lab. We largely favor the free / open source versions of many types of software even if there is a similar proprietary program. These programs can be easily downloaded for free from the Internet wherever you are, and will run identically on Mac / Windows / Linux. Their successive versions are also usually completely backwards compatible. All means that if you need to explain to someone else how to do something very specific us…

spm_hlu

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T17:38:32+00:00

======1L SPM+HLU ====== LOCATION: Shelf K * 3g Potassium Acetate * 0.2g Raffinose Pentahydrate * 50mg Ura * 50mg His * 100mg Leu * Get a 2000mL flask and put apprioprate amount of Potassium Acetate and Raffinose Pentahydrate in flask. Then locate URA, HIS, and LEU located on top shelf in Amino Acid bins then add appropriate amount of each to flask.

sporulation_of_diploids

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T17:23:48+00:00

Sporulation of Diploids --- Ryla Cantergiani 2020/03/30 17:07 Overview ---------- Sporulation is how you generate haploid yeast strains from diploid yeast strains. An example of this would be to generate haploid deletion strains from a diploid strain from the het dip (heterozygous diploid) deletion collection.

ss-glucuronidase

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T15:10:25+00:00

ß-Glucuronidase --- Olivia Heath 2018/02/07 15:01 ---------- Description ß-Glucuronidase Formula: ‎N/A [Link to MSDS] Location: 4°C Fridge UNM Barcode: 117085 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number: IC15228401 Order Link:

start

Anonymous (anonymous@undisclosed.example.com) — 2019-11-05T10:10:51+00:00

McCormick Lab Wiki Welcome to the wiki page for the McCormick Lab. Here you will find details about our protocols, equipment, and reagents, and lots of other resources that will help you in the lab. If you are a lab member and don't have an account to edit this site, talk to Mark or Christine to get an account.

starvation_for_autophagy_imaging

Anonymous (anonymous@undisclosed.example.com) — 2019-07-19T11:32:59+00:00

Day 0 1. Overnight yeast to be imaged. 2. Make serial dilutions - add 3.3 mL to tube A, 3 mL to tube B, 2.7 to tube C. Add sample to tube A and vortex. Take 300 uL from tube A, add to tube B, vortex. Take 300 uL from tube B, add to tube C, vortex.

steps_to_hiring_students

Anonymous (anonymous@undisclosed.example.com) — 2018-04-12T13:45:52+00:00

Steps to Hiring Students 1. Email Mark with: * work study type (federal/state) * amount ($$) * semester allocation (spring/fall/summer/year) * UNM banner ID number * UNM email address * current UNM employment status 2. Go to job posting (provided by Mark via email with direct link). Electronically sign hiring document through

stir_bars

Anonymous (anonymous@undisclosed.example.com) — 2020-04-06T23:06:56+00:00

Stir Bars --- Ryla Cantergiani 2020/04/06 22:51 Description ---------- Typically white-colored, elongated plastic pellets with magnets inside. These are used for stirring contents of containers (flasks, beakers, etc.) through the power of electro-magnetism on stirplates, instead of having something stick out of the top to stir.

stls_and_assembly

Anonymous (anonymous@undisclosed.example.com) — 2024-12-18T17:00:34+00:00

Download Lazy Susan Download Truss Diamond Carousel Holder Download Carousel Part 1 Download Carousel Part 2 Download Carousel Part 3 Download Carousel Part 4

stratalinking_plates

Anonymous (anonymous@undisclosed.example.com) — 2023-04-09T11:37:05+00:00

Stratalinking Plates Overview When sterilizing plates, typically worm plates, other protocols have been used in our lab. If you are unsure if this protocol is appropriate for your experiment, ask someone else for help. This protocol is generally completed two days after

streaking_yeast_strains

Anonymous (anonymous@undisclosed.example.com) — 2018-06-26T14:18:45+00:00

Streaking Yeast Plates: 1) Clean benchtop with 70% ethanol and start Bunsen Burner. 2) Label YPD plates appropriately. 3) Pull appropriate yeast strains from freezer (see protocol on Wiki). 4) Open strain tube and scrape a pen-tip sized cluster of yeast with a sterile pipet tip.

stripping_buffer

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:55:34+00:00

Stripping Buffer --- Ryla Cantergiani 2020/06/11 15:44 Description ---------- Stripping buffer is used in Western blots to remove primary and secondary antibodies from membranes so they may be re-probed. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

submitting_research_grades_at_the_end_of_semester

Anonymous (anonymous@undisclosed.example.com) — 2018-12-10T13:31:11+00:00

Fastinfo Information Quick rundown: * Login to my.unm.edu * Look for Faculty or employee tab * Enter loboweb * Select semester & class * Submit grades, make sure date follows format exactly.

sulfuric_acid_70

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T12:44:52+00:00

Sulfuric Acid (70%) --- Olivia Heath 2018/02/07 12:42 ---------- Description Sulfuric Acid (70%) Formula: ‎H2SO4 [Link to MSDS] Location: 7- Under Hood UNM Barcode: 117078 Notes and Warning: Order Information ---------- Vendor: Fisher Scientific Catalog Number:

syringe_5_ml

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:02:34+00:00

5 mL Syringe --- Ryla Cantergiani 2020/04/20 21:01 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

syringe_10_ml

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:03:23+00:00

10 mL Syringe --- Ryla Cantergiani 2020/04/20 21:02 Description ---------- Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

syringe_filter

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T20:59:56+00:00

Syringe Filter --- Ryla Cantergiani 2020/04/20 20:58 Description ---------- Not DMSO-safe syringe filters. Enter description of the equipment, please link any protocols or reagent pages that use it. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

tae

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:20:01+00:00

TAE --- Ryla Cantergiani 2020/06/09 16:57 Description ---------- TAE is made up of tris base, acetate, and EDTA. We use three concentrations in lab: 1X, 10X, and 50X. We use 1X TAE when running gels. Enter description of reagent here. Please link any protocols that this reagent is used in.

taq_dna_polymerase_kit

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T13:26:19+00:00

Taq DNA Polymerase Kit --- Olivia Heath 2018/02/20 13:24 ---------- Description 4 x 250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 Formula: N/A [Link to MSDS] Location: -20°C Freezer UNM Barcode: 117096 Notes and Warning:

tbe

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:35:45+00:00

TBE --- Ryla Cantergiani 2020/06/11 15:30 Description ---------- TBE buffer is made of tris-base, boric acid, and EDTA. We have pre-made 1X and 10X solutions on shelf Q. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

te

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:24:39+00:00

TE --- Ryla Cantergiani 2020/06/09 17:13 Description ---------- TE is made of Tris-HCl and EDTA. We keep 10X TE on Shelf O. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

temed

Anonymous (anonymous@undisclosed.example.com) — 2020-04-28T02:06:56+00:00

TEMED --- Ryla Cantergiani 2020/04/28 02:01 Description ---------- Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

template_equipment_page

Anonymous (anonymous@undisclosed.example.com) — 2019-01-04T14:25:07+00:00

Instructions: Please use this template in order to add a new piece of equipment. This includes freezers and fridges as well as glassware. Anything that the lab buys once and does not get used up is considered equipment. In order to use this template to make a new page, click

template_media_recipe

Anonymous (anonymous@undisclosed.example.com) — 2018-02-08T12:19:40+00:00

Instructions: When adding a new media recipe, please make sure you link to all of the appropriate reagents and equipment needed in the recipe. This includes autoclaves and pH meters and anything else you could possibly link from another page. In order to use this template to make a new page, click

template_protocol

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T17:07:48+00:00

Instructions: When adding a new protocol, please make sure you link to all of the appropriate reagents and equipment needed. This includes autoclaves and pH meters and anything else you could possibly link from another page. In order to use this template to make a new page, click

template_reagent_page

Anonymous (anonymous@undisclosed.example.com) — 2018-01-19T11:56:55+00:00

Instructions: When adding a new reagent, please make sure that it has been added to the Inventory in the dropbox and into the UNM ERM system. In order to use this template to make a new page, click “Edit this page” on the right and copy everything below this into the new wiki page in order to conserve formatting.

templates

Anonymous (anonymous@undisclosed.example.com) — 2019-10-13T22:01:28+00:00

Wiki Templates When making a new wiki page, please use the templates below. Template Reagent Page Template Media Recipe Template Protocol Template Equipment Page

tetracycline_hydrochloride

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T15:53:48+00:00

Tetracycline Hydrochloride --- Olivia Heath 2018/02/07 15:51 ---------- Description Tetracycline Hydrochloride Formula: ‎C22H25ClN2O8 [Link to MSDS] Location: 4°C Fridge UNM Barcode: 117097 Notes and Warning: Order Information ---------- Vendor: VWR Catalog Number:

tetrad_digestion_and_dissection

Anonymous (anonymous@undisclosed.example.com) — 2020-03-30T17:05:29+00:00

Tetrad Digestion and Dissection --- Ryla Cantergiani 2020/03/30 16:43 Overview ---------- This protocol describes dissection of yeast tetrads. In our lab, we primarily use tetrad dissection for constructing strains for genetic and biochemical experiments. Materials

thawing_worms_strains

Anonymous (anonymous@undisclosed.example.com) — 2024-05-08T12:49:18+00:00

Thawing Worm Strains Procedure ---------- 1. Find out what worm strains you need to thaw. 2. Get 1 OP-50 seeded large (10cm) NGM plate per strain to thaw worms on. 3. Label plates with the strain name, initials, date, and any other additional information about the strain.

tobramycin

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:08:29+00:00

Tobramycin --- Ryla Cantergiani 2020/05/07 17:59 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

tough_spots

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T21:28:25+00:00

Tough Spots --- Ryla Cantergiani 2020/04/20 21:26 Description ---------- Tough spots are used for labelling tubes. We have two sizes: 1/2“ and 3/8”. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

trace_metals_solution

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:41:24+00:00

Trace Metals Solution --- Ryla Cantergiani 2020/06/09 17:40 Description ---------- Enter description of reagent here. Please link any protocols that this reagent is used in. [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

treating_slides_with_concanavalina

Anonymous (anonymous@undisclosed.example.com) — 2019-06-26T22:15:46+00:00

Treating Slides with ConcanavalinA 1) ConcanavalinA is stored in the -20°C freezer in the box labeled “ConcanavalinA.” Allow it to thaw. 2) Clean glass microscope slides with 95% ethanol and a kimwipe. Clean coverslips as well. Only handle the edges.

trichloroacetic_acid

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:05:08+00:00

Trichloroacetic Acid --- Olivia Heath 2018/02/07 12:45 ---------- Description Trichloroacetic Acid Formula: ‎C2HCl3O2 [Link to MSDS] Location: 4°C Refrigerator UNM Barcode: 117074 Notes and Warning: Wear Gloves Order Information ---------- Vendor: VWR Catalog Number:

tris-buffer_acetic_acid_edta_tae

Anonymous (anonymous@undisclosed.example.com) — 2017-10-27T10:39:11+00:00

242 g Tris Base (powder) 57 ml HOAc – glacial acetic acid solution (under hood in cabinet) N.B: GLACIAL ACETIC ACID is severely corrosive. Use under hood, avoid any contact with skin, eyes, etc. If you are not completely sure how to work with glacial acetic acid safely go over this with your mentor before using it.

tris

Anonymous (anonymous@undisclosed.example.com) — 2020-06-11T15:42:27+00:00

TRIS --- Olivia Heath 2018/02/07 12:49 --- Ryla Cantergiani 2020/04/28 02:10 ---------- Description TRIS aka TRIS Base. We use TRIS when making TAE stocks. We keep a pre-made 1M Tris solution at pH=7 on Shelf Q. Formula: C4H11NO3 [Link to MSDS] Location: Shelf ?? UNM Barcode: 117018

tris_hydrochloride

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:12:03+00:00

Tris Hydrochloride --- Olivia Heath 2018/02/07 13:09 ---------- Description Tris Hydrochloride. We keep a pre-made 1M solution on Shelf O. Formula: ‎C4H12ClNO3 [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117019, 117059 Notes and Warning: DO NOT USE FOR TAE

trizol

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T14:09:25+00:00

TRIzol --- Olivia Heath 2018/02/20 14:02 ---------- Description Invitrogen TRIzol Reagent, 100 ml Formula: C16H8Cl2FN5O Location: 4°C Fridge UNM Barcode: 117098, 117100 Notes and Warning: Order Information ---------- Vendor: Fisher Scientific Catalog Number:

tryptone

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:26:22+00:00

Tryptone --- Olivia Heath 2018/02/07 13:13 ---------- Description Tryptone Formula: ‎N/A [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117017, 117060, 117061, 117062 Notes and Warning: Order Information ---------- Vendor: Genesee / Apex Catalog Number:

tween_20

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:26:44+00:00

Tween 20 --- Olivia Heath 2018/02/07 13:17 ---------- Description Tween 20 Formula: ‎C58H114O26 [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117021 Notes and Warning: Order Information ---------- Vendor: Bio-world.com / bioPLUS Catalog Number: 42030016-2 ( 730223 )

undergrad._students

Anonymous (anonymous@undisclosed.example.com) — 2019-07-24T13:41:40+00:00

UNM Specific Programs Non-UNM Programs

unm_specific_programs

Anonymous (anonymous@undisclosed.example.com) — 2019-07-24T13:42:58+00:00

MARC Program - Program offered to students 2 years away from graduation. Monthly stipend provided for participating in program with two years dedicated to a research project under a mentor. UPN Program - Summer program for students wanting to conduct research. Summer stipend is awarded to those who enter the program and are able to work on a research project that they will present at the end of the sum…

uracil

Anonymous (anonymous@undisclosed.example.com) — 2020-05-07T18:47:46+00:00

Uracil --- Olivia Heath 2018/02/07 13:19 ---------- Description Uracil Formula: ‎C4H4N2O2 [Link to MSDS] Location: WW- Chemical Shelf UNM Barcode: 117049 Notes and Warning: Order Information ---------- Vendor: Bioworld Catalog Number: 42190000-1 ( 705833 )

useful_resources

Anonymous (anonymous@undisclosed.example.com) — 2019-10-07T11:58:32+00:00

UNM Resources Steps to hiring students Putting in a Service Request Reserving BRF 218 Filling out your timesheet UNM Department of Safety and Risk Services Hazardous Waste Pick Up Class Resources Helpful sites for UNM classes Lab Resources Journal Club Methods YouTube video on basics of bash Beginner's Tutorial to Unix

using_the_ph_meter

Anonymous (anonymous@undisclosed.example.com) — 2020-04-02T23:49:53+00:00

Using the pH Meter --- Ryla Cantergiani 2020/04/02 23:32 Overview ---------- The pH meter is used when you need to measure the pH of something or make something of a certain pH. It is very important you know what you're doing before you've used it - there has been trouble with carelessness in the past! The instructions are hung on the wall behind the pH meter - please use them.

uv_stratalinker

Anonymous (anonymous@undisclosed.example.com) — 2019-02-27T20:35:03+00:00

UV Stratalinker --- Christine Robbins 2019/02/27 20:23 Description ---------- The UV Stratalinker is used to kill bacteria that are used as the food source in C. elegans lifespan experiments. When treating worms with drugs, especially antibiotics, the bacteria must be killed before proceeding with experimetns.

vacuum_filter

Anonymous (anonymous@undisclosed.example.com) — 2020-04-20T22:10:12+00:00

Vacuum Filter --- Ryla Cantergiani 2020/04/20 21:41 Description ---------- Vacuum filters are used when vacuum sterilizing. We have two sizes of vacuum filters: 50 mL and 250 mL. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

vacuum_pump

Anonymous (anonymous@undisclosed.example.com) — 2019-02-28T15:49:42+00:00

Vacuum Pump --- Christine Robbins 2019/02/28 15:18 Description ---------- The vacuum pump is used in order to aspirate out supernatants, vacuum filter reagents, or for the vacuum manifold that is used in the Mini Prep protocol. Location:Fume Hood Notes and Warning:

velveteen

Anonymous (anonymous@undisclosed.example.com) — 2019-02-28T15:18:34+00:00

Velveteen --- Christine Robbins 2019/02/28 15:05 Description ---------- Velveteen is utilized in the replica plating of yeast cells. The velveteen's texture allows the cells to be copied to a new plate exactly without morphing the appearance or location of a colony.

vortexer

Anonymous (anonymous@undisclosed.example.com) — 2019-02-28T15:04:59+00:00

Vortexer --- Christine Robbins 2019/02/28 14:58 Description ---------- The vortexer is used in order to resuspend cells in culture or to mix drug containing solutions. Location:Below shelf CC Notes and Warning: This machine does not require a lot of force to run. Just a gentle touch with your sample of I will start the vortex.

washing_lab_dishes

Anonymous (anonymous@undisclosed.example.com) — 2018-11-14T15:58:16+00:00

Washing Lab Dishes --- Christine Robbins 2018/11/14 15:39 Description ---------- Whenever making media, all glassware, stir bars, and lab scoops must be promptly cleaned. If you have not cleaned up your mess, you have not completed the protocol. Notes and Warning:

weigh_boats

Anonymous (anonymous@undisclosed.example.com) — 2020-04-23T23:22:08+00:00

Weigh Boats --- Ryla Cantergiani 2020/04/23 23:14 Description ---------- Weigh boats are small plastic receptacles used to measure out materials for making media. They are disposable. We have three sizes: small, medium, and large. But in most protocols, you will use a medium weigh boat.

working_in_the_lab_for_credit_hours

Anonymous (anonymous@undisclosed.example.com) — 2019-07-24T13:49:56+00:00

BIOM410 - Undergraduate research offered to students for upper-division credit. 3 Hours spent in the lab is equal to 1 credit hour Biochemistry Students BIOC499 - Undergraduate research at any level for 1-3 credit hours. 3 Hours spent in the lab is equal to 1 credit hour.

worm_freezing_solution

Anonymous (anonymous@undisclosed.example.com) — 2020-06-09T17:34:38+00:00

Worm Freezing Solution --- Christine Robbins 2018/10/01 14:01 --- Ryla Cantergiani 2020/06/09 17:30 Description ---------- As it's name suggests, worm freezing solution is added to worms before freezing them in the -80ºC incubator to ensure the survival of L1 worms. Location:

worm_incubators

Anonymous (anonymous@undisclosed.example.com) — 2020-04-06T20:27:28+00:00

Worm Incubators --- Ryla Cantergiani 2020/04/06 18:20 Description ---------- Most strains of C. elegans need to be maintained at 20°C, and so we store our strains in incubators. Make sure your plates are labeled when placed inside the incubators. [Insert image of equipment here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of

worm_lifespan

Anonymous (anonymous@undisclosed.example.com) — 2018-10-01T13:37:56+00:00

Worm Lifespans --- Christine Robbins 2018/10/01 13:14 Overview ---------- As always, read the entire protocol before starting a lifespan or scoring a lifespan and if anything is not clear, ASK! We perform worm lifespans in order to measure changes lifespan caused by RNAi, mutations, or compounds to C. elegans.

worm_microscopes

Anonymous (anonymous@undisclosed.example.com) — 2019-02-28T14:56:54+00:00

Worm Microscopes --- Christine Robbins 2019/02/28 14:50 Description ---------- Worm microscopes are used in order to score worm lifespans, for assaying egg laying or thrashing, or for any other C. elegans related experiment. Location: Equipment maintenance:

yeast_colony_pcr

Anonymous (anonymous@undisclosed.example.com) — 2018-11-08T12:26:55+00:00

Yeast Colony PCR [Insert Signature here. You must be signed in to do this] Overview ---------- This is an easy PCR protocol for 10ul reactions. HOW DOES PCR WORK? Please, please, please know this if you are using this technique. Start HERE Materials

yeast_dissection_microscopes

Anonymous (anonymous@undisclosed.example.com) — 2020-10-05T18:12:21+00:00

Description ---------- The Yeast Dissection Scopes are used for dissecting budding yeast from the mother during Replicative Lifespan (RLS) experiments. Location: Notes and Warning: Be wary of the the needle used for the dissection because it can puncture the media of the yeast plate being worked with if one is not paying attention. Always check the needle before use.

yeast_extract_powder

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:28:40+00:00

Yeast Extract Powder --- Olivia Heath 2018/02/07 13:22 ---------- Description Yeast Extract Power Formula: ‎N/A [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117020, 117063, 117064, 117065, 117066 Notes and Warning: Order Information ---------- Vendor:

yeast_nitrogen_base_without_amino_acids_ammonium_sulfate

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T16:28:59+00:00

Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate --- Olivia Heath 2018/02/07 13:24 ---------- Description Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate Formula: ‎N/A [Link to MSDS] Location: MM- Chemical Shelf UNM Barcode: 117022 Notes and Warning:

yeast_pcr

Anonymous (anonymous@undisclosed.example.com) — 2018-05-04T15:16:43+00:00

Yeast PCR Set up To begin, pull the plates of the strains you will be PCRing from the 4C fridge. * Open the MM PCR folder on the desktop. * Open the file called PCR Template. * Save as your initials, the strains you are testing, and the date.

yeast_transformation_episomal

Anonymous (anonymous@undisclosed.example.com) — 2018-06-27T13:53:05+00:00

Episomal Yeast Transformation: 1) Clean benchtop with 70% ethanol and start Bunsen Burner. 2) Gather following materials: Overnight Strains To Be Used YPD Media 10 X TE 50% PEG 3550 Salmon Sperm DNA 1 M LiOAc 0.1 M LiOAc 3) Ensure that heat blocks (95C and 42C) are turned on and heated.

yeastar_rna_kit

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T14:17:42+00:00

YeaStar RNA Kit --- Olivia Heath 2018/02/20 14:13 ---------- Description YeaStar RNA Kit Formula: N/A Location: OO Kit Shelf UNM Barcode: 117051 Notes and Warning: Order Information ---------- Vendor: Genesee Scientific Catalog Number: 11-329 Order Link

yep_media_and_variations

Anonymous (anonymous@undisclosed.example.com) — 2018-11-15T08:50:35+00:00

YEP Media and Variations --- Christine Robbins 2018/11/14 15:59 Description ---------- YEP Liquid Media is used to grow up yeast cells after a carbon source has been added. There are a number of variations including different carbon sources and drugs for selection of strains with deletions/markers.

yep_plates_and_variations

Anonymous (anonymous@undisclosed.example.com) — 2020-04-10T23:29:45+00:00

YEP and Variations --- Ryla Cantergiani 2020/04/10 22:18 Description ---------- YEP is liquid yeast culture media. YEP does not have glucose, meaning it does not have any food in it. To add food, add 2% glucose to the YEP, making it YEPD. Always label whether you have made YEP or YEPD.

yeti-_liquid_nitrogen_dewar

Anonymous (anonymous@undisclosed.example.com) — 2019-02-28T14:49:37+00:00

Yeti- Liquid Nitrogen Dewar --- Christine Robbins 2019/02/28 14:42 Description ---------- The Yeti is what we use in order to retrieve and use liquid nitrogen. Liquid nitrogen can be obtained from the BMB department in Fitz hall on the second floor (have someone show you where it is and how to use it). Liquid nitrogen is used in worm lysis protocols and for freezing bacterial strains.

zymoclean_dna_clean_concentrator

Anonymous (anonymous@undisclosed.example.com) — 2018-02-20T14:20:37+00:00

Zymoclean DNA Clean & Concentrator --- Olivia Heath 2018/02/20 14:18 ---------- Description DNA Clean & Concentrator-25, Uncapped Columns Formula: N/A Location: OO- Kit Shelf UNM Barcode: 117053 Notes and Warning: Order Information ---------- Vendor:

zymoclean_gel_dna_recovery_kit

Anonymous (anonymous@undisclosed.example.com) — 2018-02-07T14:29:11+00:00

Zymoclean Gel DNA Recovery Kit --- Olivia Heath 2018/02/07 14:26 ---------- Description Zymoclean Gel DNA Recovery Kit Formula: ‎N/A Location: OO- Kit Shelf UNM Barcode: 117052 Notes and Warning: Order Information ---------- Vendor: Genesee / Zymo