McCormick Lab Wiki

annotations_with_labelimg - created

alex_achusim (alex_achusim@undisclosed.example.com) — 2026-05-14T17:12:29+00:00

Setup Guide for Frame Annotation Syncthing and LabelImg [--- Alex Achusim 2026/05/14 17:09] Overview ---------- Enter description of the media, please link any protocols that it is used in. In this section you put why we do this protocol and how it works very generally,as well as what the protocol(s) immediately preceding this and immediately following this are (with wiki links), if applicable.

protocols - [WAM Protocols]

alex_achusim (alex_achusim@undisclosed.example.com) — 2026-05-14T17:07:05+00:00

Protocols ---------- Media and Stock Solutions ---------- Liquid Media Recipes Making Liquid Media Yeast Media YEP Media and Variations SC, SD, SD(-N), and Dropout Media Min, Min D+, Min G+ Bacteria Media LB and Variations Worm Media M9 Media Worm S-Basic Work S-Complete Worm Freezing Solution Solid Media Recipes Making Solid Media and Pouring Plates Yeast Plates YEP Plates and Variations SC, and Dropout Plates Basic Plates Basic +HLU Plates GNA Plates Bacteria Plates L…

single_worm_lysis_and_pcr

madison_otero (madison_otero@undisclosed.example.com) — 2025-09-05T14:45:04+00:00

Single Worm Lysis and PCR --- Madison Otero 2025/08/14 13:07 Overview ---------- Purpose: to amplify regions of interest of DNA. Materials ---------- PK Buffer PCR Master Mix dH2O dH2O 20 mg/mL PK 10X Buffer 10X worm buffer

labeling_a_gel

madison_otero (madison_otero@undisclosed.example.com) — 2025-09-05T13:48:32+00:00

Labeling a Gel * Make a new folder with the date, who done each thing, and what the gel is. * Put the original image in the folder. * Open with GIMP * Image > Transform > Rotate 90 ° clockwise * Tools > Selection Tools > Rectangle select

bacterial_pcr - created

madison_otero (madison_otero@undisclosed.example.com) — 2025-08-12T18:10:33+00:00

Bacterial PCR --- Madison Otero 2025/08/12 17:29 Overview ---------- PCR protocol for testing bacteria for RNAi inserts. Materials ---------- * Baxter water * 2X bacteria master mix * bacteria in liquid media * PCR primers * ice * flip top tubes

stls_and_assembly - created

alex_achusim (alex_achusim@undisclosed.example.com) — 2024-12-18T17:00:34+00:00

Download Lazy Susan Download Truss Diamond Carousel Holder Download Carousel Part 1 Download Carousel Part 2 Download Carousel Part 3 Download Carousel Part 4

fudring_worms - created

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2024-12-02T16:31:58+00:00

FUDRing Worms Adding FUDR to worm plates with proper timing and placement should prevent progeny from developing in adult worms later in the lifepspan Overall, 50uL of FUDR should be added to each plate (1 drop of 10 uL in lawn, and 4 drops of 10uL surrounding the lawn)

thawing_worms_strains - [Part Numbers]

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2024-05-08T12:49:18+00:00

Thawing Worm Strains Procedure ---------- 1. Find out what worm strains you need to thaw. 2. Get 1 OP-50 seeded large (10cm) NGM plate per strain to thaw worms on. 3. Label plates with the strain name, initials, date, and any other additional information about the strain.

1mm_levamisole_hydrochloride

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2024-02-12T15:01:39+00:00

1mm levamisole hydrochloride Izabelle Chavez Description Location:-20°C Media Recipe and Directions Directions * Aliquot 4 µL of stock solution into one flip-top tube. * Dilute with M9 to 1000 µL (1 mL). * Store at 4°C. Note: this concentration goes bad in one week.

250mm_levamisole_hydrochloride

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2024-02-12T14:58:21+00:00

250mm levamisole hydrochloride Izabelle Chavez Description Location:-20°C Notes and Warning: Wear gloves when handling solid levamisole hydrochloride. Media Recipe and Directions Directions * Mix 0.12038 g (120.38 mg) of levamisole hydrochloride in 15 mL of sterile M9.

color_coding_table.jpg - created

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2023-01-20T14:53:16+00:00

repeaterpipette-image.png - created

izabelle_chavez (izabelle_chavez@undisclosed.example.com) — 2022-11-11T16:11:36+00:00

chemical_waste_pickup_request.xlsx - created

christine_robbins (christine_robbins@undisclosed.example.com) — 2021-05-20T21:09:02+00:00

replicaplatingtool-map.png - created

aaron_romero (aaron_romero@undisclosed.example.com) — 2020-10-05T18:26:54+00:00

replicaplatingtool-image.jpg - created

aaron_romero (aaron_romero@undisclosed.example.com) — 2020-10-05T18:25:46+00:00

yeastdissectionscope-map.png - created

aaron_romero (aaron_romero@undisclosed.example.com) — 2020-10-05T18:10:31+00:00

yeastdissectionscope-image.jpg - created

aaron_romero (aaron_romero@undisclosed.example.com) — 2020-10-05T17:30:31+00:00

logo.png

christine_robbins (christine_robbins@undisclosed.example.com) — 2020-09-01T11:23:31+00:00

pipette-tips-r-new-rc-041320.png - created

ryla_cantergiani (ryla_cantergiani@undisclosed.example.com) — 2020-04-13T15:20:49+00:00

pipette-tips-rc-041320.png - created

ryla_cantergiani (ryla_cantergiani@undisclosed.example.com) — 2020-04-13T14:54:33+00:00