M9

M9 solution is used when working with worms. It is used in Freezing Worm Strains.

Location:✱ Notes and Warning: M9 should be clear in appearance; if cloudy or otherwise unusual, make a new stock. Notes and Warning: Make sure you are using the hydrated form of all the reagents. ===== Media Recipe and Directions===== Directions - 1 L - Label a 1L beaker with media, initials, and date. Add a stir bar. - Fill with 1/4 of the MilliQ H2O - Add Na2HPO4*7H2O, KH2PO4, NaCl, and MgSO4*7H2O. Stir until all solid has dissolved. - Add additional MilliQ H2O to reach the desired volume. - Pour into labeled bottles. Autoclave. Media Recipe ^ Reagents ^ 500 mL ^ 1 L ^ 2 L ^ 3 L ^ | Na2HPO4*7H20 | 2.9 g | 5.8 g | 11.6 g | 17.4 g | | potassium acetate anyhydrous (KH2PO4) | 1.5 g | 3.0 g | 6.0 g | 9.0 g | | sodium chloride (NaCl) | 2.5 g | 5.0 g | 10.0 g | 15.0 g | | magnesium sulfate (MgSO4*7H2O) | 0.125 g | 0.25 g | 0.50 g | 0.75 g | | milliQ water | 500 mL | 1000 mL | 2000 mL | 3000 mL | Storage instructions**

Store in bottles at room temperature.

Na2HPO4*7H2O P/N: Sigma-Aldrich S9390-1KG

KH2PO4 P/N: VWR BDH0268-5006

NaCl P/N: EMD SX0420-3

MgSO4*7H2O P/N: Bioworld 413000-40-1

Millipore filter P/N: Millipore SCGP505RE (<0.22um) and bottle.