6X Loading Dye

— Ryla Cantergiani 2020/06/11 16:13

6X PCR loading dye is used to stain your sample when run through gel electrophoresis. Dye allows you to keep track of how far your sample has moved through the gel. It also allows you to estimate that approximately the same amount of sample is added to each well. We use bromophenol blue and glycerol.

Add 2 μL of 6X loading dye to each well (with sample) of your PCR strip. Add directly into your sample.

[Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of “nameofreagent-image.png” . Select “Add images and other files” from above and upload your image. When the Link Settings tab pops up, select “Direct link to original”, “Align the image on the right” and “Medium Version”, then click insert. ]

Formula: [If applicable]

Link to MSDS [In order to do this, type “Link to MSDS”, highlight it and select “Add Images and other files” from above. Add the MSDS from the dropbox folder located in dropbox>mccormicklabshared>Administrative>Lab Inventory, MSDS> MSDS]

Location: Drawer 36

[Insert Image of lab location here. To do this, take the template of the lab, that is in the wiki folder, and add a red dot in the location of the reagent using InkScape. Export the image to the dropbox folder 'mccormicklab1>Wiki-Documents and Figures> Pictures for Wiki….> Reagents' With the file name of “nameofreagent-map.png”. Then, add the image by selecting “Add images and other files” from above and upload your image. When the Link Settings tab pops up, select “Direct link to original”, “Align the image on the right” and “Medium Version”, then click insert. ]

UNM Barcode:

Notes and Warning: Please use the flip-top tube labeled with a red dot. When that tube is finished, pick a new tube, and label it with a red dot. If you notice we are running low, make more or notify the tech.

• 3.75ml glycerol (30% final concentration)
• 25mg bromophenol blue (0.25% final concentration)
• dH2O to 10mL

This will normally make 30 mL of dye. Separate into 1 mL aliquots in labeled flip-top tubes.

Vendor:

Part Number:

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