50% Glucose

— Christine Robbins 2018/05/01 10:40

The stock solution of D-glucose that we keep on hand is 50% (w/v) Glucose. This is used when making a number of yeast media recipes including, YPD liquid media, YPD plates, and all of our synthetic and drop-out media recipes.

Location:Shelf L

Directions

• You will need a labeled beaker that will fit your final volume of media and a graduated cylinder
• Place a stirbar into the beaker and add about 1/4 the final volume of milliQ Water
• Turn on the stir bar and the hot plate (on low, the water should be warm, not hot or boiling)
• Weigh out the appropriate amount of D-glucose. You will have to use multiple weigh boats so please keep track of how much you have weighed.
• SLOWLY add the glucose in. I recommend adding a little at a time and coming back when the solution is clear, then adding more. This will take a while. If you just dump in the glucose, you will create a rock that will take forever to dissolve
• When all of the glucose is added, add milliQ water to the appropriate volume
• Filter sterilize the 50% glucose into labeled bottles

Media Recipe