{{ :image8.jpeg?400|}}====== Worm Freezing Solution ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 14:01//
 --- //[[rylajosephine@gmail.com|Ryla Cantergiani]] 2020/06/09 17:30//

==== Description ====
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As it's name suggests, worm freezing solution is added to worms before freezing them in the -80ºC incubator to ensure the survival of L1 worms. 


**Location:** Shelf R


**Notes and Warning:** Please note that 1M MgSO4 is added after autoclaving and the volume in the bottle will not be 1L or 2L. Be sure to add 30μL of 1M MgSO4 per 100mL in your bottle. DO NOT STORE THIS MEDIA UNTIL THE FINAL INGREDIENT HAS BEEN ADDED!

===== Media Recipe and Directions=====

**Media Recipe**                

^ Reagents                        ^  Final Volume           ||
|                                 ^ 1L             ^ **2L**  ^
| [[milliq_water|milli-Q water]]  | 710mL          | 1420mL  |
| [[sodium_chloride|NaCl]]        | 5.8g           | 11.6g   |
| 1M [[1m_potassium_phosphate_ph_6.04|KH2PO4]]                       | 50mL           | 100mL   |
| [[glycerol|Glycerol]]           | 240mL          | 480mL   |
^  **Add After Autoclaving**                               |||
| 1M MgSO4                        | 3mL            | 6mL     |

**Directions** 
  * In a labeled [[glassware|beaker]] of the appropriate size, add a stir bar and 1/4 the final volume of [[milliq_water|milli-Q water]]
  * Add the appropriate volume of the reagents listed below
  * Once mixed, transfer the solution to labeled bottles
  * [[autoclave_media|Autoclave]] the media
  * Add the remaining reagents and store at room temperature 


==== Order Information ====
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