====== Sequencing Tubes (Eurofin) ======

 --- //[[rylajosephine@gmail.com|Ryla Cantergiani]] 2020/04/20 21:05//

=====Overview=====

This protocol will outline how to submit sequencing to Eurofins using the sequencing tubes provided. 

=====Protocol=====

  - Take your PCR product and clean and concentrate it with DNA concentration kit. 
  - Dilute the DNA to the specifications based on the size of your PCR product. (For 0.3-0.8 kb, 40-80 ng/5ul per tube)
  - Dilute the primers to the pmol specified (6-20 pmol per 5ul)
  - Combine the two dilutions. 
  - Add to the sequencing tubes. Record the tube number and the sample you pipetted into the tube. 
==== Order Information ====
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^ Item  ^ Vendor  ^ Part Number  ^ Order Link  ^
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