====== Refilling Ethanol Bottles ======

 --- //[[rylajosephine@gmail.com|Ryla Cantergiani]] 2020/04/28 01:21//

===== Overview =====
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We use 70% and 95% stocks of [[200_proof_ethanol|200-proof ethanol]] to sterilize our bench spaces and several pieces of equipment in lab. Squeeze bottles of 70% and 95% are on every bench, and they are refilled as part of Friday Clean-Up tasks (or when needed).



===== Materials=====
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^ Materials           ^  
| 200 proof ethanol  |
| squeeze bottles (500 mL) |
| milliQ water |
| graduated cylinder |

=====Procedure=====
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==70% Ethanol==
  - Collect the ethanol bottles from around the lab. Use the remaining ethanol to refill any bottles possible. Do not mix 70% and 95%. Use remaining ethanol for clean off the benches or in a protocol :) (or just dump it down the sink)
  - Fill a graduated cylinder with 350 mL of 200 proof ethanol. Do this in the fume hood. Add to squirt bottle.
  - Fill the squeeze bottle up to the line with milliQ water (about 150 mL).
  - Careful shake to mix.

==95% Ethanol==
  - Collect the ethanol bottles from around the lab. Use the remaining ethanol to refill any bottles possible. Do not mix 70% and 95%. Use remaining ethanol for clean off the benches or in a protocol :) (or just dump it down the sink)
  - Fill a graduated cylinder with 475 mL of 200 proof ethanol. Do this in the fume hood. Add to squirt bottle.
  - Fill the squeeze bottle up to the line with milliQ water (about 25 mL).
  - Careful shake to mix.


=====Notes=====
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Ethanol is extremely flammable. Do NOT do this anywhere need an open flame.

=====Part Numbers=====
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Reagents
^ Reagent            ^ Part Number  ^
| 200-Proof Ethanol  | 71002-512    |
| milliQ water  | 

Equipment
^ Equipment  ^ Part Number  ^
| squeeze bottle     |         |
| graduated cylinder |         |