====== Reagents and Consumables ====== Reagents and consumables are items in the lab that are used up and need to be re-made or re-ordered. This includes all chemicals, media, and single-use lab plastics/tips/etc. ==Instructions:== When adding a new item to the reagents and consumables sheet, always create a new page below under the correct section, placing the item in the correct alphabetical order. Always use the appropriate template page when creating a page. Templates can be found [[templates|here]]. Always fill in all available information correctly and get help if you need it. As always, if you need any help or if something is not clear, please ask. ---- ====Chemcials and Reagents==== [[2-Mercaptoethanol, 55mM]] [[30% Acrylamide/Bis Solution, 29-1]] [[4-Nitrophenol]] [[40% Acrylamide/Bis Solution, 19-1]] [[5-FOA]] [[8-Azaadenine]] [[Acetic Acid, Glacial]] [[Adenine (7H-Purin-6-amine)]] [[Agarose]] [[Ammonium Persulfate]] [[Ammonium Sulfate]] [[Amphotericin B]] [[Ampicillin Sodium Salt]] [[AN-2690]] [[Apex PBS Buffer 10x Dry Pack]] [[Apex Peptone]] [[Bacto Agar]] [[Bacto Peptone]] [[Bacto Yeast Extract]] [[Bafilomycin A1]] [[BD Nutrient Broth]] [[Berberine Chloride]] [[Betaine]] [[BioWorld Agar]] [[BioWorld Nutrient Broth]] [[Boric Acid]] [[Borrelidin]] [[Bortezomib]] [[Bovine Serum Albumin]] [[Bromophenol Blue]] [[Calcium Chloride]] [[Carbenicillin]] [[CDP-Star]] [[Chloramphenicol]] [[Chloroform]] [[Chloroquine]] [[Cholesterol]] [[Clear Bath]] [[Concanavalin A]] [[Coomassie Plus Protein Assay Reagent]] [[Copper(II) Sulfate]] [[Cycloheximide]] [[D(+) Raffinose]] [[D-Glucose]] [[DAPI]] [[DEPC (Diethyl dicarbonate)]] [[DMEM, High Glucose, no L-Glutamine or Sodium Pyruvate]] [[DMSO]] [[DNA PCR Ladder]] [[dNTPs (10mM each)]] [[Doxycycline]] [[Dry Milk]] [[DTT (1,4-Dithio-DL-threitol)]] [[EDTA]] [[Epetraborole]] [[Ethanol, 200 proof]] [[Ethidium Bromide]] [[Formaldehyde]] [[Formamide]] [[FUDR]] [[FuGene HD Transfection reagent]] [[G418 Sulfate]] [[Gelatin]] [[GenClone™ Fetal Bovine Serum]] [[Glycerol]] [[Glycine]] [[Glycolic Acid]] [[HBSS]] [[Heparin Sodium Salt]] [[Himedia Agar]] [[Himedia Yeast Extract]] [[Hydrochloric Acid]] [[Hydroxychloroquine Sulfate]] [[Hygromycin B]] [[IPTG]] [[Iron(II) Sulfate]] [[Isopropanol]] [[Kanamycin Sulfate]] [[L-Alanine]] [[L-Arginine]] [[L-Aspartic Acid]] [[L-Cysteine HCL]] [[L-Glutamic Acid]] [[L-Glutamine Solution, 100X]] [[L-Glutamine]] [[L-Histidine]] [[L-Isoleucine]] [[L-Leucine]] [[L-Lysine]] [[L-Methionine]] [[L-Phenylalanine]] [[L-Proline]] [[L-Serine]] [[L-Threonine]] [[L-Tryptophan]] [[L-Tyrosine]] [[L-Valine]] [[Lamivudine]] [[Levamisole]] [[Lithium Acetate]] [[Magnesium Chloride Trace Metals]] [[Magnesium Chloride]] [[Magnesium Sulfate]] [[Maleic Acid]] [[Metformin]] [[Methylene Blue]] [[Mupirocin]] [[Myo-Inositol]] [[NEAA Solution, 100X]] [[Nourseothricin]] [[Para-Amino Benzoic Acid]] [[Penicillin-Streptomycin, 100X]] [[Phenylmethylsulfonyl fluoride]] [[PIPES]] [[PMSF]] [[PNPP]] [[Polyethylene Glycol]] [[Potassium Acetate]] [[Potassium Chloride]] [[Potassium Hydroxide]] [[Potassium Phosphate]] [[Proteinase K]] [[QuickExtract DNA Extraction solution]] [[Rapamycin]] [[Salmon Sperm DNA]] [[Sodium (meta)arsenite]] [[Sodium Acetate]] [[Sodium Azide]] [[Sodium Chloride]] [[Sodium Citrate tribasic]] [[Sodium dodecyl sulfate]] [[Sodium Hydrogen Phosphate]] [[Sodium Hydroxide]] [[Sodium Hypochlorite]] [[Sodium Phosphate]] [[Sodium Pyruvate, 100 mM]] [[Sterile Baxter Water]] [[Sucrose]] [[Sulfuric Acid]] [[Sunrise Agar]] [[Sunrise Agar]] [[Synapto Red, FM4-64]] [[TEMED]] [[Tetracycline Hydrochloride]] [[Texas Red]] [[Thapsigargin]] [[TO-PRO -3 Iodide, 1mM]] [[Tobramycin]] [[Trichloroacetic Acid]] [[Tripotassium citrate]] [[Tris Base]] [[Tris-HCl]] [[Triton X-100]] [[Trizol Reagent]] [[Trypan Blue Stain (0.4%)]] [[Trypsin-EDTA]] [[Tryptone]] [[Tween-20]] [[Uracil]] [[Urea]] [[Xylene Cyanol FF]] [[Yeast Nitrogen Base]] [[Zidovudine]] [[Zymolyse]] [[β-Glucuronidase]] ---- ====Media==== ===Yeast=== ===Worm=== ===Bacteria=== ===Cell Culture=== ---- ====Buffers and Stock Solutions==== ---- ====Kits==== [[Apex PCR Kit]] [[Chromatin Condensation/Dead Cell Apoptosis Kit]] [[Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit]] [[Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)]] [[DIG Nucleic Acid Detection Kit]] [[DIG Oligonucleotide 3’-End Labeling Kit]] [[Dual Luciferase Reporter Assay System]] [[Gateway LR Clonase II]] [[Live or Dead™ Cell Viability Assay Kit]] [[Luminescent Detection Kit]] [[Neon™ Transfection System 100 µL Kit]] [[One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli]] [[pENTR™/D-TOPO™ Cloning Kit]] [[Q5 HiFi PCR Kit]] [[Qiagen Midiprep Kit]] [[Qiagen Miniprep Kit]] [[Qiagen PCR Kit]] [[Yeast Strain Pulling]] [[Zymo DNA Clean and Concentrator Kit]] [[Zymo Gel DNA Recovery Kit]] [[Zymo Yeast Plasmid Miniprep]] ---- ====Plastics and Consumables==== [[1.5mL Flip Top Tubes]] [[1.8ml Self-Standing Cryovial]] [[12-Well Cell Culture Plates] [[15mL Conical Tubes]] [[24-Well Cell Culture Plates]] [[24-well Plates, non-coated]] [[250mL Vacuum Filter]] [[384-well qPCR Plates]] [[50mL Conical Tubes]] [[50mL High-Speed Centrifuge Tubes]] [[50mL Ultracentrifuge Tubes]] [[50mL Vacuum Filter]] [[8-well PCR Strips]] [[96-well Black Plates]] [[96-well plates, Black-wall, Clear-bottom, TC treated]] [[96-well Plates, clear non-coated]] [[Bacteria Loop]] [[BioScreen]] [[Blot Paper]] [[Cell Spreader]] [[Corks and Cork Borer]] [[Cotton Tipped Applicator]] [[Cryo-Labels]] [[Cuvette]] [[Eurofins Sequencing]] [[FACS Tubes]] [[Foil Plate Seal]] [[Glass Beads]] [[Gloves]] [[Hybond Membrane]] [[Kim Wipes]] [[Lab Printer]] [[Lab Tape]] [[Large Plates]] [[Microscope Cameras and Accessories]] [[Microscope Slides]] [[Microscope Slides]] [[Microscope Slides]] [[Mini Worm Plates]] [[Northern Blot Boxes]] [[Parafilm]] [[Pipette Aide]] [[Pipette Tips]] [[Plastic Wrap]] [[Reagent trough]] [[Repeater Pipette Tips]] [[RNAse Free Spray]] [[RNAse Free Water]] [[Rubber Bands]] [[Screw Top Tubes]] [[Serological Pipettes]] [[Small Worm Plates]] [[Syringe Filters]] [[Syringe Needles]] [[Syringes]] [[TC Treated Dishes]] [[TC Treated Flasks]] [[Toothpicks]] [[Vacuum Pump]] [[Weigh Boats]] [[Worm Microscopes]] [[Worm Picks]] [[Yeast Dissecting Scopes]] [[Zymo RNA Extraction Kit]] ---- ====Misc. Consumables==== [[Lab Maintenance]] [[Safety Equpiment]] [[Cleaning Supplies]] [[Office Supplies]] [[Tools]] ---- [[200 proof Ethanol]] [[1.5 mL Flip-Top Tubes]] [[4-Nitrophenol]] [[5-FOA (5-Fluoroorotic Acid anhydrous)]] [[6X PCR Loading Dye]] [[8-Azaadenine]] [[15 mL Conical Tubes]] [[24-Well Plates, non-treated]] [[50 mL Conical Tubes]] [[96-Well Plates, non-treated]] [[96-Well Plates, Black]] [[96-Well Plate Stickers]] [[394-Well Plates]] [[1000 μL Tips for Vacuum Pump]] [[Acrylamide/Bis Solution]] [[Adenine (7H-Purin-6-amine)]] [[Adhesive Foil]] [[Agar, Bacteriological]] [[Agarose]] [[Ammonium Sulfate]] [[Amphotericin B]] [[Ampicillin]] [[Apex DNA Ladder I]] [[Apex PCR DNA Ladder]] [[Bacto Peptone]] [[Baxter Water]] [[Berberine Chloride]] [[β-Glucuronidase]] [[Bioscreen Plates]] [[Borrelidin]] [[Bovine Serum Albumin]] [[Bromophenol blue]] [[C-his dropout powder]] [[C-leu dropout powder]] [[C-lys dropout powder]] [[C-met dropout powder]] [[C-met thr cys dropout powder]] [[C-met cys dropout powder]] [[C-met thr dropout powder]] [[C-trp dropout powder]] [[Calcium Chloride]] [[Carbenicillin Disodium Salt]] [[CDP-Star]] [[Chloramphenicol]] [[Chloroform]] [[Cholesterol]] [[Citric acid monohydrate]] [[Clear Nail Polish]] [[Concanavalin A]] [[Copper (II) sulfate pentahyrate]] [[Coomassie Plus Protein Assay Reagent]] [[Cycloheximide]] [[D-(+)-Raffinose Pentahydrate]] [[D-Glucose]] [[DAPI]] [[DEPC (Diethyl dicarbonate)]] [[Di-Sodium hydrogen phosphate anhydrous]] [[Dig Blocking Solution]] [[Dig Detection Buffers]] [[Dig Wash Buffer]] [[DMSO]] [[DMSO-Safe Syringe Filter]] [[dNTP mix 10µM each]] [[Doxycycline]] [[DTT (1,4-Dithio-DL-threitol)]] [[DualLuciferase Reporter Assay]] [[EDTA]] [[Ethidium Bromide]] [[Formamide]] [[FUDR]] [[G418 Sulfate]] [[GC10 Competent Cells E. Coli]] [[Gel-Loading Tips]] [[Gelatin]] [[Glacial Acetic Acid]] [[Glass Rods]] [[Gloves]] [[Glycerol]] [[Glycine]] [[Glycolic Acid]] [[Heparin sodium salt]] [[High-Speed Centrifuge Tubes]] [[Hybridization Buffer]] [[Hybridization Wash]] [[Hydrochloric Acid]] [[Hygromycin]] [[IPTG]] [[Iron (II) sulfate heptahydrate]] [[Isopropyl Alcohol]] [[Kanamycin Sulfate]] [[Kimwipes]] [[L-Alanine]] [[L-Arginine Base]] [[L-Asparagine]] [[L-Aspartic Acid]] [[L-Cysteine HCL Monobasic]] [[L-Glutamic Acid]] [[L-Glutamine]] [[L-Histidine HCL]] [[L-Isoleucine]] [[L-Leucine]] [[L-Lysine HCL]] [[L-Methionine]] [[L-Phenylalanine]] [[L-Proline]] [[L-Serine]] [[L-Threonine]] [[L-Tryptophan]] [[L-Tyrosine]] [[L-Valine]] [[Lab Tape]] [[Large Plates (10cm)]] [[Levamisole]] [[Lithium Acetate dihydrate]] [[Magnesium Chloride Hexahydrate]] [[Magnesium Sulfate]] [[Maleic Acid Buffer]] [[Manganese(II) chloride tetrahydrate]] [[Metformin Hydrochloride]] [[Methylene Blue]] [[Microscope Slides and Covers]] [[MilliQ Water]] [[Mini Worm Plates]] [[Mupirocin]] [[Myo-Inositol]] [[Nourseothricin]] [[Nutrient Broth]] [[Para-AminoBenzoic Acid (PABA)]] [[Parafilm]] [[Pasteur Pipettes]] [[PCR Strips (8-Well)]] [[PEG Transformation Solution]] [[Peptone]] [[PIPES]] [[Platinum Wire]] [[PMSF]] [[PNPP]] [[Polyethylene Glycol]] [[Polysome Lysis Buffer]] [[Potassium acetate anhydrous]] [[β-Glucuronidase]] [[Potassium Chloride]] [[Potassium Citrate]] [[Potassium Hydroxide]] [[Potassium Phosphate]] [[Proteinase K]] [[Qiagen Plasmid Midi Kit]] [[Rapamycin]] [[Reagent Troughs]] [[Repeater Pipette Tips]] [[RNAse FREE Spray]] [[RNAse FREE Water]] [[Rubber Bands]] [[Salmon Sperm]] [[Screw Top Tubes]] [[SC dropout powder]] [[Serological Pipettes]] [[Sequencing Tubes (Eurofins)]] [[Sodium Acetate]] [[Sodium Azinde]] [[Sodium Chloride]] [[Sodium Hydroxide]] [[Sodium Hypochlorite 6%]] [[Sodium Phosphate]] [[Stripping Buffer]] [[Sulfuric Acid (70%)]] [[Syringe, 5 mL]] [[Syringe, 10 mL]] [[Syringe Filter]] [[TAE]] [[TBE]] [[Taq DNA Polymerase Kit]] [[TE]] [[TEMED]] [[Tetracycline Hydrochloride]] [[Tobramycin]] [[Tough Spots]] [[Trace Metals Solution]] [[Trichloroacetic Acid]] [[Triphotassium citrate monohydrate]] [[TRIS]] [[Tris HydroChloride]] [[Triton X-100]] [[TRIzol]] [[Tryptone]] [[Tween 20]] [[Uracil]] [[Vacuum Filter]] [[Weigh Boats]] [[Worm Freezing Solution]] [[Yeast Extract Powder]] [[Yeast Nitrogen Base without Amino Acids & Ammonium Sulfate]] [[YeaStar RNA Kit]] [[Zymoclean DNA Clean & Concentrator]] [[Zymoclean Gel DNA Recovery Kit]]