====== Pulling Bacterial Strains ======

 --- //[[rylajosephine@gmail.com|Ryla Cantergiani]] 2020/04/13 14:38//

===== Overview =====
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We have our bacterial strains frozen in the -80C freezer. When you need to use a strain, you'll have to pull it and grow it up first. The bacteria will take time to grow up, so plan ahead when organizing your experiments.


===== Materials=====
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^ Materials                ^   
| frozen bacterial strain  |  
| [[lb_and_variations|LB]] (liquid or plates) |
| [[pipette_tips|pipette tips]] |

=====Procedure=====
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  - Check the MM-BACTERIA-STRAINS spreadsheet to find the MM# of your bacteria
  - Depending on what you need the bacteria for, you can grow it up on LB plates or in LB liquid media. Labels your plates/tubes.
  - Ethanol and wipe down your bench. Turn on a flame.
  - Using the polar bear gloves, get your bacterial strain out of the -80C freezer.
  - Using a pipette tip (P10 or P20 work best), get a small bit of bacteria out of the tube, and gently place it onto your plate/into your liquid LB. If using a plate, let the bacteria dry before streaking out. If using liquid LB, vortex to mix.
  - Place in the 37C incubator to grow. Plates can go in the 37 in lab. Tubes need to go in the shaking 37C in CRF.


=====Notes=====
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=====Part Numbers=====
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