======= Protocols =======

----


=====Media and Stock Solutions=====

----
====Liquid Media Recipes====

[[Making Liquid Media]]

===Yeast Media===

[[YEP Media and Variations]]

[[SC, SD, SD(-N), and Dropout Media]]

[[Min, Min D+, Min G+]]

===Bacteria Media===

[[LB and Variations]]

===Worm Media===

[[M9 Media]]

[[Worm S-Basic]]

[[Work S-Complete]]

[[Worm Freezing Solution]]

====Solid Media Recipes====

[[Making Solid Media and Pouring Plates]]

===Yeast Plates===

[[YEP Plates and Variations]]

[[SC, and Dropout Plates]]

[[Basic Plates]]

[[Basic +HLU Plates]]

[[GNA Plates]]

===Bacteria Plates===

[[LB Plates and Variations]]

===Worm Plates===

[[NGM Plates and Variations]]

[[Small NGM Plates]]

[[Stratalinking Plates]]

====Stock Solutions====

[[5mg/ml Cholesterol]]

[[50% Glucose]]

[[80% Glycerol]]

[[100mg/ml Ampicillin]]

[[1M NaOH]]

[[50% NaOH]]

[[10M NaOH]]

[[SPM +HLU]]

[[50% PEG]]

[[10x TE]]

[[1M LiOAC]]

[[0.5M LiOAC]]

[[0.1M LiOAC]]

[[50x TAE]]

[[1x TAE]]

[[0.5M EDTA]]

[[1M CaCl2]]

[[1M MgSO4]]

[[1M Potassium Phosphate pH 6.04]]

[[95% EtOH]]

[[70% EtOH]]

[[100mM FUDR (1000x)]]

[[10mM FUDR (100x)]]

[[250mM Levamisole hydrochloride]]

[[1mM Levamisole hydrochloride]]

[[2% Agarose]]

[[20mg/ml Proteinase K]]

[[100mg/ml Carbenicillin]]

[[1M IPTG]]

[[100mg/ml G418]]

[[10mg/ml EtBr]]

[[SC and Dropout Powders]]


=====Yeast Protocols=====

----

[[Freezing Yeast Strains]]

[[Overnight Yeast Strains]]

[[Pulling Yeast from the Freezer]]

[[Yeast Transformation (Episomal)]]

[[High Efficiency Yeast Transformation]]

[[Dual Luciferase Assay]]

[[Yeast Lifespan]]

[[Crossing Yeast Strains]]

[[Sporulation of Diploids]]

[[Tetrad Digestion and Dissection]]

[[Replica Plating]]

[[Scoring Tetrads]]

[[Yeast Colony PCR]]

[[Streaking Yeast Strains]]

[[Treating slides with ConcanavalinA]]

[[Starvation for Autophagy Imaging]]

[[Protein Extraction]]

[[Gateway Cloning]]

=====Bacterial Protocols=====

----

[[Freezing Bacterial Stocks]]

[[Pulling Bacterial Strains from Freezer]]

[[Making OP-50 Stock]]

[[Bacterial Transformation]]

[[Midi Prep]]

[[Mini Prep]]

[[ Vacuum Manifold]]

[[Overnight Bacterial Strains]]

[[Streaking out Bacterial Strains]]

[[Bacterial PCR]]

[[Gateway Multisite LR Cloning]]


=====Worm Protocols=====

----

[[Seeding NGM Plates]]

[[Freezing Worm Stocks]]

[[Thawing worms Strains]]

[[Worm Lifespan]]

[[Bleaching Worms]]

[[Chunking Worms]]

[[Practicing with Worms on the Scope]]

[[Single Worm Lysis and PCR]]

[[Mounting Worms for Imaging]]

[[FUDRing Worms]]

=====Cell Culture Protocols=====

----

[[Cell Culture Aseptic Technique Important Notes and Points to Avoid Contamination]]

[[mouse embryonic fibroblast growth media]]

[[MEF Freeze/Thaw procedure]]

[[MEF CRISPR KO]]

[[Confirming CRISPR deletion and detecting if frameshift using Benchling]]

[[MEF QuickExtract DNA extraction procedure]]

[[MEF subculturing]]

[[MEF subculturing 24-well plate]]

[[Cellomics CX7 MEF drug exposure for ATT BIOQUEST LIVE/DEAD assay]]

[[MEF Viafect Plasmid Transfection]]

[[MEF Image Data Labeling in R]]

=====Misc. Protocols=====

----

[[Lab Rules to Live By]]

[[Diluting and freezing Primers]]

[[Gel Electrophoresis]]

[[Labeling a Gel]]

[[Using the pH Meter]]

[[Filter Sterilizing Stocks]]

[[Washing Lab Dishes]]

[[Autoclave Glassware]]

[[Autoclave Media]]

[[Adding Materials to the ERM]]

[[Hypoxia Chamber Setup]]

[[Refilling Ethanol Bottles]]

[[Batch Scale Bar Worm Photos Using ImageJ]]

[[Adding Drugs to Plates]]

[[Adding PEG to Plates]]

=====WAM Protocols=====

----

[[STLs and assembly]]

[[Annotations with LabelImg]]