====== PEG Transformation Solution ====== --- //[[rylajosephine@gmail.com|Ryla Cantergiani]] 2020/06/08 21:48// ==== Description ==== ---- Polyethylene glycol (PEG) transformation solution is used to mediate transformations. We keep a 50% PEG solution on Shelf O. {{ :genericchemicalsforreagenttemplate.png?direct&400|}} [Insert image of reagent here. To do this, take a picture of the reagent on the shelf, save it on to the shared computer 'mccormicklab1>Wiki-Documents and Figures> Pictures for WIki with a file name of "nameofreagent-image.png" . Select "Add images and other files" from above and upload your image. When the Link Settings tab pops up, select "Direct link to original", "Align the image on the right" and "Medium Version", then click insert. ] **Formula:** [If applicable] **{{ :msds-ammoniumsulfate.pdf |Link to MSDS}}** [In order to do this, type "Link to MSDS", highlight it and select "Add Images and other files" from above. Add the MSDS from the dropbox folder located in dropbox>mccormicklabshared>Administrative>Lab Inventory, MSDS> MSDS] **Location:**Shelf AAA [Insert Image of lab location here. To do this, take the template of the lab, that is in the wiki folder, and add a red dot in the location of the reagent using InkScape. Export the image to the dropbox folder 'mccormicklab1>Wiki-Documents and Figures> Pictures for Wiki....> Reagents' With the file name of "nameofreagent-map.png". Then, add the image by selecting "Add images and other files" from above and upload your image. When the Link Settings tab pops up, select "Direct link to original", "Align the image on the right" and "Medium Version", then click insert. ] **UNM Barcode:** **Notes and Warning:** Please indicate here if the reagent requires any special handling or storage. ==== 50% PEG Transformation Solution Recipe ==== ^ Materials ^ | polyethylene glycol (powder) | | diH2O | | vacuum filter | | beaker | | [[magnetic_hot_plates|magnetic hot plate]] | Directions - Place 50 g polyethylene glycol powder in beaker - Add 30-40 mL diH2O - Heat and stir until totally clear (this will take a long time) - Dilute to 100 mL with diH20 (wait about 40 minutes and until it becomes clear) - Transfer to a 125 mL bottle using a vacuum filter. ==== Order Information ==== ---- **Vendor:** **Part Number:** **Order Link:**