Materials:
  * colored lab tape
  * glass slides 
  * cover glass
  * fast-dry clear nail polish 
  * microscope slide book
  * 2% agarose
  * 70% EtOH
  * Kim Wipes
Steps: 
  - Clean and dry slides and cover glass to be used. Note: only 70% EtOH and Kim Wipes should be used to clean these.
  - Prepare two glass slides with tape on each end of both slide if not already prepared. Note: for standard 1 mm wide slide, 3 mm colored lab tape is sufficient (3 layer wrap).
  - Place a slide in center of the two tape prepped slides. 
  - Add 20 μL of 2% agarose to center of middle slide. 
  - Place one slide atop the drop, with sides leaning on the taped ends surrounding the drop. 
  - Press down the top slide to smash the agarose pad. 
  - Flip the slide with the agarose pad over.
  - Add 1 drop (~2 μL) of levamisole to the center of the agarose pad. 
  - Place worm(s) in the levamisole drop.
  - Place cover glass on the drop, dropping from side angle. Note: ideally there should be no bubbles made here.
  - Stick the cover glass to the glass slide by placing a drop of quick-dry nail polish in each corner of the cover glass. Note: sealing the cover glass is only necessary if you are reimaging these worms later. 
  - Place the slide on a scope with the cover glass side facing down. 
  - Circle around each worm by marking the glass slide.
  - Image the worms with the cover glass side facing down.