=====Overview===== To perform DNA extraction on MEF cells, use QuickExtract. QuickExtract will detach and destroy the adherent MEFs cultured on a tissue culture plate, and after this, ~10-minute protocol, the DNA in the mixture will be ready for PCR. =====Protocol===== - Culture MEFs in a 96-well or a 24-well plate. Using larger plates will result in the use of more reagents but is doable as well. - Retrieve QuickExtract aliquot from the -20 freezer, adjust 42*C heat block to 65*C. - In the BSL-2 hood, aspirate off media - Add Enough QuickExtract to the well to cover the plate's surface area - With a micropipette, scrape the plate to ensure the removal and destruction of the MEF cells. Mix the QuickExtract liquid around the plate with the pipette. - Move the liquid into a microcentrifuge tube. - Vortex for 15 seconds. - Place on 65*C heat block for 6 min. - Vortex for 15 seconds. - Place on 95*C heat block for 2 min. - Once cooled to room temperature, your sample is now ready to PCR.