====== M9 ======

==== Description ====
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M9 solution is used when working with worms. It is used in [[freezing_worm_stocks|Freezing Worm Strains]].

 {{ :genericchemicalsforreagenttemplate.png?direct&400|}} 
{{:m9.jpeg?400|}}


**Location:✱
{{:m9_lab_map.png?400|}}



**Notes and Warning:** M9 should be clear in appearance; if cloudy or otherwise unusual, make a new stock.

**Notes and Warning:** Make sure you are using the hydrated form of all the reagents.

===== Media Recipe and Directions=====

**Directions - 1 L** 
  - Label a 1L beaker with media, initials, and date. Add a stir bar.
  - Fill with 1/4 of the MilliQ H2O
  - Add Na2HPO4*7H2O, KH2PO4, NaCl, and MgSO4*7H2O. Stir until all solid has dissolved.
  - Add additional MilliQ H2O to reach the desired volume.
  - Pour into labeled bottles. Autoclave.

**Media Recipe** 

^ Reagents                               ^ 500 mL   ^ 1 L      ^ 2 L      ^ 3 L      ^
| Na2HPO4*7H20                           | 2.9 g    | 5.8 g    | 11.6 g   | 17.4 g   |
| potassium acetate anyhydrous (KH2PO4)  | 1.5 g    | 3.0 g    | 6.0 g    | 9.0 g    |
| sodium chloride (NaCl)                 | 2.5 g    | 5.0 g    | 10.0 g   | 15.0 g   |
| magnesium sulfate (MgSO4*7H2O)         | 0.125 g  | 0.25 g   | 0.50 g   | 0.75 g   |
| milliQ water                           | 500 mL   | 1000 mL  | 2000 mL  | 3000 mL  |     


**Storage instructions**

Store in bottles at room temperature.


=====Part Numbers=====
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Na2HPO4*7H2O  P/N: Sigma-Aldrich S9390-1KG

KH2PO4 P/N: VWR BDH0268-5006

NaCl P/N: EMD SX0420-3

MgSO4*7H2O P/N: Bioworld 413000-40-1

Millipore filter P/N: Millipore SCGP505RE (<0.22um) and bottle.