{{ :image6.jpeg?400|}}====== 80% Glycerol ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 14:19//

==== Description ====
----

The stock solution of Glycerol that we keep on hand is 80% (v/v) Glycerol. This is used when making a number of yeast media recipes including, YPG liquid media, YPG plates, and is used in freezing down yeast and bacterial cultures.


**Location:**Shelf L


===== Media Recipe and Directions=====

**Directions** 

  * You will need a labeled [[glassware|beaker]] that will fit your final volume of media and a graduated cylinder
  * Place a stirbar into the beaker and add about 50mL (500mL final volume) or 100mL (1L final volume) [[milliq_water|Milli-Q water]]
  * Turn on the stir bar and the hot plate (on low, the water should be warm, not hot or boiling)
  * Add the appropriate volume of Glycerol. Glycerol is incredibly viscous so be patient when pouring it out
  * When all of the glycerol is added, add milliQ water to the appropriate volume
  * Allow it to stir until incorporated. If it is not, you will see a division between water and glycerol in the beaker
  * [[filter_sterilizing_stocks|Filter sterilize]] the 80% glycerol into labeled bottles

**Media Recipe**
                  

^ Reagents               ^  Final Volume          ||
|                        ^ 500mL          ^ 1L     ^
| [[glycerol|Glycerol]]  | 400mL          | 800mL  |