{{ :image1.jpeg?400|}}====== 5mg/ml Cholesterol ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 13:49//

==== Description ====
----

5mg/ml cholesterol is used for making NGM plates for worms. 


**Location:** 4ºC Fridge


**Notes and Warning:** This solution is dissolved in ethanol, please make sure all labels are covered in clear tape so that they do not run off. If they do start to run, re-label 

This can take a while to fully dissolve.

===== Media Recipe and Directions=====

**Directions** 
  * Add a small volume of [[200_proof_ethanol|ethanol]] to a labeled [[50ml_centrifuge_tubes|50mL tube]] (about 4mL)
  * Add [[cholesterol|cholesterol]] to the tube
  * Add [[200_proof_ethanol|ethanol]] to 50mL and vortex until mixed
  * Sterilize the cholesterol by [[filter_sterilizing_stocks|filter sterilization]]
  * Wrap the lid in parafilm
  * Store the new labeled tube at 4ºC

**Media Recipe**
                 

^              ^ Final Volume  ^
^ Reagent      ^ 50mL          ^
| [[cholesterol|Cholesterol]]  | 250mg         |