{{ ::image4.jpeg?400|}}====== 50% NaOH ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 15:02//

==== Description ====
----

50% NaOH is used in LB liquid media. 


**Location:**Shelf L

**Warnings** WEAR GLOVES!


===== Media Recipe and Directions=====

**Directions** 

  * Add 1/4 the final volume [[milliq_water|milli-Q]] water into a labeled [[glassware|beaker]] with a stir bar
  * Add the appropriate amount of [[sodium_hydroxide|NaOH(s)]] to the beaker and mix until dissolved
  * Add water to the final volume
  * Transfer to a labeled bottle when dissolved

**Media Recipe**
                  

^ Reagents                   ^  Final Volume                ||
|                            ^ 250mL          ^ 500mL  ^ 
| [[sodium_hydroxide|NaOH]]  | 125g            | 250g    |