{{ :image7.jpeg?400|}}
====== 50% Glucose ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/05/01 10:40//

==== Description ====
----

The stock solution of D-glucose that we keep on hand is 50% (w/v) Glucose. This is used when making a number of yeast media recipes including, YPD liquid media, YPD plates, and all of our synthetic and drop-out media recipes. 


**Location:**Shelf L


===== Media Recipe and Directions=====

**Directions** 

  * You will need a labeled beaker that will fit your final volume of media and a graduated cylinder
  * Place a stirbar into the beaker and add about 1/4 the final volume of milliQ Water
  * Turn on the stir bar and the hot plate (on low, the water should be warm, not hot or boiling)
  * Weigh out the appropriate amount of D-glucose. You will have to use multiple weigh boats so please keep track of how much you have weighed. 
  * SLOWLY add the glucose in. I recommend adding a little at a time and coming back when the solution is clear, then adding more. This will take a while. If you just dump in the glucose, you will create a rock that will take forever to dissolve
  * When all of the glucose is added, add milliQ water to the appropriate volume
  * [[filter_sterilizing_stocks|Filter sterilize]] the 50% glucose into labeled bottles

**Media Recipe**
                  

^ Reagents                 ^  Final Volume                       ||||
|                          ^ 500mL          ^ 1L    ^ 2L   ^ 3L     ^
| [[d-glucose|D-Glucose]]  | 250g           | 500g  | 1kg  | 1.5kg  |