====== 2% Agarose ======

Izabelle Chavez

==== Description ====
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**Location:**drawer 46

**Notes and Warning:** Please indicate here if the reagent requires any special handling or storage.



===== Media Recipe and Directions=====

  - Measure out 400 mg agarose in small weight boat with small scale. 
  - Pour agarose into 50 mL flask. 
  - Add Baxter water up to 20 mL line of flask.
  - Slosh to mix agarose and water.
  - Microwave flask without foil for 15 seconds.
  - Swirl solution, if there is still undissolved agarose microwave in 15 second intervals until all is dissolved.

**Media Recipe**

For mounting worms, 20mL is sufficient. This calls for 400 mg agarose in 20 mL Baxter water. 

                  

^ Reagents      ^  Final Volume                             ||||
|               ^ 5 mL           ^ 10 mL   ^ 15 mL   ^ 20 mL   ^
| Agarose       | 100 mg         | 200 mg  | 300 mg  | 400 mg  |
| Baxter Water  | 5 mL           | 10 mL   | 15 mL   | 20 mL   |
|                                                          |||||

**Aliquoting and Storage Guide**

You can store 1 mL agarose per flip top tube. Note: move quickly to store since this solution will solidify.

Place the tube top before rewarming and rewarm each tube at 65-75°C with 3rd spare heat block. Prep the heat block overnight if not already set.