====== 1M LiOAc ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 16:10//

==== Description ====
----
1M LiOAC is used in a number of transformation protocols including episomal transformations and high efficiency transformations 


**Location:**Shelf L

===== Media Recipe and Directions=====

**Directions** **Directions**
  * Get a [[glassware|beaker, and bottles]] and place a stir bar in the beaker
  * Label all bottles and beaker with 1M LiOAc, your initials and the date
  * Add [[milliq_water|MilliQ Water]] to about 1/4 of your final volume 
  * With the bar stiring, add the reagents listed below
  * After all of the reagents are incorporated, fill the beaker to the final volume
  * Pour the media into labeled bottles (only fill to the highest marked volume on the bottle. Example: a 500mL bottle only has gradations to 400mL, so only add 400mL media) 
  * With the caps partialy screwed on (you should be able to wiggle them without them coming off), label with autoclave tape
  * [[autoclaves|Autoclave]] 
  * When cool, screw caps on completely and store


**Media Recipe**

^ Reagents  ^  Final Volume               |||
|           ^ 500mL          ^ 1L   ^ 2L    ^
| [[lithium_acetate_dihydrate|LiOAC]]     | 33g            | 66g  | 132g  |