====== 0.5M LiOAc ======

 --- //[[celiser12@gmail.com|Christine Robbins]] 2018/10/01 16:54//

==== Description ====
----

0.5M LiOAc is used in a number of transformation protocols including episomal and high efficiency transformations. 


**Location:**Shelf L


===== Media Recipe and Directions=====

**Directions** 
  * In order to make 0.5M stock of LiOAc, dilute from the [[1m_lioac|1M stock]] by adding the appropriate volume into a [[50ml_centrifuge_tubes|50mL tube]]
  * Fill to final volume with baxter water
  * Cap and store at room temperature 

**Media Recipe**


^ Reagents                    ^  Final Volume                       ^^
|                             ^ 25mL          ^ 50mL   ^ 
| [[1m_lioac|1M LiOAc]]         |   12.5mL           | 25mL   |
| Baxter water                  |   12.5mL             | 25mL    |