Seeding Large NGM Plates * 2 600 mL Beakers * 200% EtOH * OP-50 * Hockey Stick - Put 50-100ml 200% EtOH and OP-50 in separate beakers. - Put on flame for sterilization and put hockey stick over flame until hot. - Dip hockey stick in 50-100ml EtOH and over flame again(wait for flame). - Then dip sterilized hockey stick in OP-50. - Streak the dipped OP-50 hockey stick in a cross (+) on lg NGM Plates. *Don't need to sterilize every time but when in doubt DO IT. Clean Up -With left over OP-50 add bleach and cover with foil. Let sit for a couple minutes then you can put it down the sink Seeding Small NGM Plates * 1 600 mL Beaker * 1 Repeater Pipette * 1 mL Comb Tips * OP-50 - Place a 1 mL comb tip on the repeater pipette. - Set the repeater pipette to administer 50 μL. - Add 10-20 mL Lb + OP-50 in the 600 mL beaker. - Collect OP-50 from 600 mL beaker with the repeater pipette. - Stack plates in stacks of five. - Add 50 μL OP-50 to each plate starting from the bottom plate in the stack. Clean Up -With left over OP-50 add bleach and cover with foil. Let sit for a couple minutes then you can put it down the sink